Probing expression of E-selectin using CRISPR-Cas9-mediated tagging with HiBiT in human endothelial cells

Summary: E-selectin is expressed on endothelial cells in response to inflammatory cytokines and mediates leukocyte rolling and extravasation. However, studies have been hampered by lack of experimental approaches to monitor expression in real time in living cells. Here, NanoLuc Binary Technology (Na...

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Hlavní autoři: Lydia Ogrodzinski, Simon Platt, Joelle Goulding, Cameron Alexander, Tracy D. Farr, Jeanette Woolard, Stephen J. Hill, Laura E. Kilpatrick
Médium: Článek
Jazyk:English
Vydáno: Elsevier 2023-07-01
Edice:iScience
Témata:
On-line přístup:http://www.sciencedirect.com/science/article/pii/S2589004223013093