Implementation of a Design of Experiments to Improve Periplasmic Yield of Functional ScFv Antibodies in a Phage Display Platform

Implementation of a Design of Experiments to Improve Periplasmic Yield of Functional ScFv Antibodies in a Phage Display Platform

Purpose: Production of functional recombinant antibody fragments in the periplasm of E. coli isa prerequisite step to achieve sufficient reagent for preclinical studies. Thus, the cost-effectiveand lab-scale production of antibody fragments demands the optimization of culture conditions.Methods: The...

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Bibliographic Details
Main Authors: Marjan Abri Aghdam, Mohammad Reza Tohidkia, Elham Ghamghami, Asadollah Ahmadikhah, Morteza Khanmahamadi, Behzad Baradaran, Ahad Mokhtarzadeh
Format: Article
Language:English
Published: Tabriz University of Medical Sciences 2022-05-01
Series:Advanced Pharmaceutical Bulletin
Subjects:
single-chain variable fragment (scfv)
phage display
response surface methodology
periplasmic expression
optimization
d-optimal design
Online Access:https://apb.tbzmed.ac.ir/PDF/apb-12-583.pdf

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https://apb.tbzmed.ac.ir/PDF/apb-12-583.pdf

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