TSA-PACT: a method for tissue clearing and immunofluorescence staining on zebrafish brain with improved sensitivity, specificity and stability

TSA-PACT: a method for tissue clearing and immunofluorescence staining on zebrafish brain with improved sensitivity, specificity and stability

Abstract For comprehensive studies of the brain structure and function, fluorescence imaging of the whole brain is essential. It requires large-scale volumetric imaging in cellular or molecular resolution, which could be quite challenging. Recent advances in tissue clearing technology (e.g. CLARITY,...

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Bibliographic Details
Main Authors: Kang Wang, Yuxin Yu, Yinhui Xu, Yingzi Yue, Fang Zhao, Wenyang Feng, Yijie Duan, Weicheng Duan, Jingjing Yue, Zhiyun Liao, Peng Fei, Hui Sun, Bo Xiong
Format: Article
Language:English
Published: BMC 2023-05-01
Series:Cell & Bioscience
Subjects:
PACT
CLARITY
Zebrafish
Immunofluorescence
Tyramide signal amplification
Tissue clearing
Online Access:https://doi.org/10.1186/s13578-023-01043-1

Internet

https://doi.org/10.1186/s13578-023-01043-1

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