A portable immunosensor provides sensitive and rapid detection of Borrelia burgdorferi antigen in spiked blood
Abstract There are no assays for detecting B. burgdorferi antigen in blood of infected Lyme disease individuals. Here, we provide proof-of-principle evidence that we can quantify B. burgdorferi antigen in spiked blood using a portable smartphone-based fluorescence microscope that measures immunoaggl...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-05-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-34108-9 |
| _version_ | 1797827538033049600 |
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| author | Sangsik Kim Kamalika Samanta Brandon T. Nguyen Samantha Mata-Robles Luciana Richer Jeong-Yeol Yoon Maria Gomes-Solecki |
| author_facet | Sangsik Kim Kamalika Samanta Brandon T. Nguyen Samantha Mata-Robles Luciana Richer Jeong-Yeol Yoon Maria Gomes-Solecki |
| author_sort | Sangsik Kim |
| collection | DOAJ |
| description | Abstract There are no assays for detecting B. burgdorferi antigen in blood of infected Lyme disease individuals. Here, we provide proof-of-principle evidence that we can quantify B. burgdorferi antigen in spiked blood using a portable smartphone-based fluorescence microscope that measures immunoagglutination on a paper microfluidic chip. We targeted B. burgdorferi OspA to develop a working prototype and added examples of two antigens (OspC and VlsE) that have diagnostic value for discrimination of Lyme disease stage. Using an extensively validated monoclonal antibody to OspA (LA-2), detection of OspA antigen had a broad linear range up to 100 pg/mL in 1% blood and the limit of detection (LOD) was 100 fg/mL (= 10 pg/mL in undiluted blood), which was 1000 times lower than our target of 10 ng/mL. Analysis of the two other targets was done using polyclonal and monoclonal antibodies. OspC antigen was detected at LOD 100 pg/mL (= 10 ng/mL of undiluted blood) and VlsE antigen was detected at LOD 1–10 pg/mL (= 0.1–1 ng/mL of undiluted blood). The method is accurate and was performed in 20 min from sample to answer. When optimized for detecting several B. burgdorferi antigens, this assay may differentiate active from past infections and facilitate diagnosis of Lyme disease in the initial weeks of infection, when antibody presence is typically below the threshold to be detected by serologic methods. |
| first_indexed | 2024-04-09T12:49:55Z |
| format | Article |
| id | doaj.art-d9d908b39d76464d82ed5951cd2ca52f |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-09T12:49:55Z |
| publishDate | 2023-05-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-d9d908b39d76464d82ed5951cd2ca52f2023-05-14T11:17:40ZengNature PortfolioScientific Reports2045-23222023-05-0113111010.1038/s41598-023-34108-9A portable immunosensor provides sensitive and rapid detection of Borrelia burgdorferi antigen in spiked bloodSangsik Kim0Kamalika Samanta1Brandon T. Nguyen2Samantha Mata-Robles3Luciana Richer4Jeong-Yeol Yoon5Maria Gomes-Solecki6Department of Biomedical Engineering, The University of ArizonaDepartment of Microbiology, Immunology and Biochemistry, University of Tennessee Health Science CenterCollege of Medicine, The University of ArizonaDepartment of Biomedical Engineering, The University of ArizonaDepartment of Microbiology, Immunology and Biochemistry, University of Tennessee Health Science CenterDepartment of Biomedical Engineering, The University of ArizonaDepartment of Microbiology, Immunology and Biochemistry, University of Tennessee Health Science CenterAbstract There are no assays for detecting B. burgdorferi antigen in blood of infected Lyme disease individuals. Here, we provide proof-of-principle evidence that we can quantify B. burgdorferi antigen in spiked blood using a portable smartphone-based fluorescence microscope that measures immunoagglutination on a paper microfluidic chip. We targeted B. burgdorferi OspA to develop a working prototype and added examples of two antigens (OspC and VlsE) that have diagnostic value for discrimination of Lyme disease stage. Using an extensively validated monoclonal antibody to OspA (LA-2), detection of OspA antigen had a broad linear range up to 100 pg/mL in 1% blood and the limit of detection (LOD) was 100 fg/mL (= 10 pg/mL in undiluted blood), which was 1000 times lower than our target of 10 ng/mL. Analysis of the two other targets was done using polyclonal and monoclonal antibodies. OspC antigen was detected at LOD 100 pg/mL (= 10 ng/mL of undiluted blood) and VlsE antigen was detected at LOD 1–10 pg/mL (= 0.1–1 ng/mL of undiluted blood). The method is accurate and was performed in 20 min from sample to answer. When optimized for detecting several B. burgdorferi antigens, this assay may differentiate active from past infections and facilitate diagnosis of Lyme disease in the initial weeks of infection, when antibody presence is typically below the threshold to be detected by serologic methods.https://doi.org/10.1038/s41598-023-34108-9 |
| spellingShingle | Sangsik Kim Kamalika Samanta Brandon T. Nguyen Samantha Mata-Robles Luciana Richer Jeong-Yeol Yoon Maria Gomes-Solecki A portable immunosensor provides sensitive and rapid detection of Borrelia burgdorferi antigen in spiked blood Scientific Reports |
| title | A portable immunosensor provides sensitive and rapid detection of Borrelia burgdorferi antigen in spiked blood |
| title_full | A portable immunosensor provides sensitive and rapid detection of Borrelia burgdorferi antigen in spiked blood |
| title_fullStr | A portable immunosensor provides sensitive and rapid detection of Borrelia burgdorferi antigen in spiked blood |
| title_full_unstemmed | A portable immunosensor provides sensitive and rapid detection of Borrelia burgdorferi antigen in spiked blood |
| title_short | A portable immunosensor provides sensitive and rapid detection of Borrelia burgdorferi antigen in spiked blood |
| title_sort | portable immunosensor provides sensitive and rapid detection of borrelia burgdorferi antigen in spiked blood |
| url | https://doi.org/10.1038/s41598-023-34108-9 |
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