A new approach to characterize cardiac sodium storage by combining fluorescence photometry and magnetic resonance imaging in small animal research
Abstract Cardiac myocyte sodium (Na+) homoeostasis is pivotal in cardiac diseases and heart failure. Intracellular Na+ ([Na+]i) is an important regulator of excitation–contraction coupling and mitochondrial energetics. In addition, extracellular Na+ ([Na+]e) and its water-free storage trigger collag...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2024-01-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-024-52377-w |
| _version_ | 1797274831417245696 |
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| author | Martin Christa Franziska Dithmar Tobias Weinaus Michael Kohlhaas Anahi-Paula Arias-Loza Michelle Hofmann Ibrahim A. Elabyad Fabian T. Gutjahr Christoph Maack Wolfgang R. Bauer |
| author_facet | Martin Christa Franziska Dithmar Tobias Weinaus Michael Kohlhaas Anahi-Paula Arias-Loza Michelle Hofmann Ibrahim A. Elabyad Fabian T. Gutjahr Christoph Maack Wolfgang R. Bauer |
| author_sort | Martin Christa |
| collection | DOAJ |
| description | Abstract Cardiac myocyte sodium (Na+) homoeostasis is pivotal in cardiac diseases and heart failure. Intracellular Na+ ([Na+]i) is an important regulator of excitation–contraction coupling and mitochondrial energetics. In addition, extracellular Na+ ([Na+]e) and its water-free storage trigger collagen cross-linking, myocardial stiffening and impaired cardiac function. Therefore, understanding the allocation of tissue Na+ to intra- and extracellular compartments is crucial in comprehending the pathophysiological processes in cardiac diseases. We extrapolated [Na+]e using a three-compartment model, with tissue Na+ concentration (TSC) measured by in vivo 23Na-MRI, extracellular volume (ECV) data calculated from T1 maps, and [Na+]i measured by in vitro fluorescence microscopy using Na+ binding benzofuran isophthalate (SBFI). To investigate dynamic changes in Na+ compartments, we induced pressure overload (TAC) or myocardial infarction (MI) via LAD ligation in mice. Compared to SHAM mice, TSC was similar after TAC but increased after MI. Both TAC and MI showed significantly higher [Na+]i compared to SHAM (around 130% compared to SHAM). Calculated [Na+]e increased after MI, but not after TAC. Increased TSC after TAC was primarily driven by increased [Na+]i, but the increase after MI by elevations in both [Na+]i and [Na+]e. |
| first_indexed | 2024-03-07T15:04:43Z |
| format | Article |
| id | doaj.art-d9de801c74594940856816ea78bd9281 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-07T15:04:43Z |
| publishDate | 2024-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-d9de801c74594940856816ea78bd92812024-03-05T18:58:55ZengNature PortfolioScientific Reports2045-23222024-01-0114111210.1038/s41598-024-52377-wA new approach to characterize cardiac sodium storage by combining fluorescence photometry and magnetic resonance imaging in small animal researchMartin Christa0Franziska Dithmar1Tobias Weinaus2Michael Kohlhaas3Anahi-Paula Arias-Loza4Michelle Hofmann5Ibrahim A. Elabyad6Fabian T. Gutjahr7Christoph Maack8Wolfgang R. Bauer9Comprehensive Heart Failure Center, University and University Hospital WürzburgComprehensive Heart Failure Center, University and University Hospital WürzburgComprehensive Heart Failure Center, University and University Hospital WürzburgComprehensive Heart Failure Center, University and University Hospital WürzburgComprehensive Heart Failure Center, University and University Hospital WürzburgComprehensive Heart Failure Center, University and University Hospital WürzburgComprehensive Heart Failure Center, University and University Hospital WürzburgExperimental Physics 5, University WürzburgComprehensive Heart Failure Center, University and University Hospital WürzburgComprehensive Heart Failure Center, University and University Hospital WürzburgAbstract Cardiac myocyte sodium (Na+) homoeostasis is pivotal in cardiac diseases and heart failure. Intracellular Na+ ([Na+]i) is an important regulator of excitation–contraction coupling and mitochondrial energetics. In addition, extracellular Na+ ([Na+]e) and its water-free storage trigger collagen cross-linking, myocardial stiffening and impaired cardiac function. Therefore, understanding the allocation of tissue Na+ to intra- and extracellular compartments is crucial in comprehending the pathophysiological processes in cardiac diseases. We extrapolated [Na+]e using a three-compartment model, with tissue Na+ concentration (TSC) measured by in vivo 23Na-MRI, extracellular volume (ECV) data calculated from T1 maps, and [Na+]i measured by in vitro fluorescence microscopy using Na+ binding benzofuran isophthalate (SBFI). To investigate dynamic changes in Na+ compartments, we induced pressure overload (TAC) or myocardial infarction (MI) via LAD ligation in mice. Compared to SHAM mice, TSC was similar after TAC but increased after MI. Both TAC and MI showed significantly higher [Na+]i compared to SHAM (around 130% compared to SHAM). Calculated [Na+]e increased after MI, but not after TAC. Increased TSC after TAC was primarily driven by increased [Na+]i, but the increase after MI by elevations in both [Na+]i and [Na+]e.https://doi.org/10.1038/s41598-024-52377-w |
| spellingShingle | Martin Christa Franziska Dithmar Tobias Weinaus Michael Kohlhaas Anahi-Paula Arias-Loza Michelle Hofmann Ibrahim A. Elabyad Fabian T. Gutjahr Christoph Maack Wolfgang R. Bauer A new approach to characterize cardiac sodium storage by combining fluorescence photometry and magnetic resonance imaging in small animal research Scientific Reports |
| title | A new approach to characterize cardiac sodium storage by combining fluorescence photometry and magnetic resonance imaging in small animal research |
| title_full | A new approach to characterize cardiac sodium storage by combining fluorescence photometry and magnetic resonance imaging in small animal research |
| title_fullStr | A new approach to characterize cardiac sodium storage by combining fluorescence photometry and magnetic resonance imaging in small animal research |
| title_full_unstemmed | A new approach to characterize cardiac sodium storage by combining fluorescence photometry and magnetic resonance imaging in small animal research |
| title_short | A new approach to characterize cardiac sodium storage by combining fluorescence photometry and magnetic resonance imaging in small animal research |
| title_sort | new approach to characterize cardiac sodium storage by combining fluorescence photometry and magnetic resonance imaging in small animal research |
| url | https://doi.org/10.1038/s41598-024-52377-w |
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