Hyperoxia but not high tidal volume contributes to ventilator-induced lung injury in healthy mice
Abstract Background Mechanical ventilation is a supportive therapy used to maintain respiratory function in several clinical and surgical cases but is always accompanied by lung injury risk due to improper treatment. We investigated how tidal volume and oxygen delivery would contribute independently...
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| Format: | Article |
| Language: | English |
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BMC
2023-09-01
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| Series: | BMC Pulmonary Medicine |
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| Online Access: | https://doi.org/10.1186/s12890-023-02626-x |
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| author | Yong Xu Yu Li Da Zhai Chen Yan Jingyan Liang Taiga Ichinomiya Tetsuya Hara Chiaki Inadomi Tao-Sheng Li |
| author_facet | Yong Xu Yu Li Da Zhai Chen Yan Jingyan Liang Taiga Ichinomiya Tetsuya Hara Chiaki Inadomi Tao-Sheng Li |
| author_sort | Yong Xu |
| collection | DOAJ |
| description | Abstract Background Mechanical ventilation is a supportive therapy used to maintain respiratory function in several clinical and surgical cases but is always accompanied by lung injury risk due to improper treatment. We investigated how tidal volume and oxygen delivery would contribute independently or synergistically to ventilator-induced lung injury (VILI). Methods Under general anesthesia and tracheal intubation, healthy female C57BL/6 N mice (9 weeks old) were randomly ventilated for 2 h by standard (7 ml/kg) or high (14 ml/kg) tidal volume at positive end-expiratory pressure (PEEP) of 2 cmH2O, with room air, 50% O2 (moderate hyperoxia), or 100% O2 (severe hyperoxia); respectively. Mice were sacrificed 4 h after mechanical ventilation, and lung tissues were collected for experimental assessments on lung injury. Results Compared with the healthy control, severe hyperoxia ventilation by either standard or high tidal volume resulted in significantly higher wet-to-dry lung weight ratio and higher levels of IL-1β and 8-OHdG in the lungs. However, moderate hyperoxia ventilation, even by high tidal volume did not significantly increase the levels of IL-1β and 8-OHdG in the lungs. Western blot analysis showed that the expression of RhoA, ROCK1, MLC2, and p-MLC2 was not significantly induced in the ventilated lungs, even by high tidal volume at 2 cmH2O PEEP. Conclusion Severe hyperoxia ventilation causes inflammatory response and oxidative damage in mechanically ventilated lungs, while high tidal volume ventilation at a reasonable PEEP possibly does not cause VILI. |
| first_indexed | 2024-03-10T22:22:39Z |
| format | Article |
| id | doaj.art-d9f0d6b1ec5d4c07975d07518b508706 |
| institution | Directory Open Access Journal |
| issn | 1471-2466 |
| language | English |
| last_indexed | 2024-03-10T22:22:39Z |
| publishDate | 2023-09-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Pulmonary Medicine |
| spelling | doaj.art-d9f0d6b1ec5d4c07975d07518b5087062023-11-19T12:14:21ZengBMCBMC Pulmonary Medicine1471-24662023-09-012311810.1186/s12890-023-02626-xHyperoxia but not high tidal volume contributes to ventilator-induced lung injury in healthy miceYong Xu0Yu Li1Da Zhai2Chen Yan3Jingyan Liang4Taiga Ichinomiya5Tetsuya Hara6Chiaki Inadomi7Tao-Sheng Li8Department of Stem Cell Biology, Atomic Bomb Disease Institute, Nagasaki UniversityDepartment of Anesthesiology, The Second Affiliated Hospital of Nanchang UniversityDepartment of Stem Cell Biology, Atomic Bomb Disease Institute, Nagasaki UniversityDepartment of Stem Cell Biology, Atomic Bomb Disease Institute, Nagasaki UniversityInstitute of Translational Medicine, Medical College, Yangzhou UniversityDepartment of Anesthesiology and Intensive Care Medicine, Nagasaki University Graduate School of Biomedical SciencesDepartment of Anesthesiology and Intensive Care Medicine, Nagasaki University Graduate School of Biomedical SciencesDepartment of Anesthesiology and Intensive Care Medicine, Nagasaki University Graduate School of Biomedical SciencesDepartment of Stem Cell Biology, Atomic Bomb Disease Institute, Nagasaki UniversityAbstract Background Mechanical ventilation is a supportive therapy used to maintain respiratory function in several clinical and surgical cases but is always accompanied by lung injury risk due to improper treatment. We investigated how tidal volume and oxygen delivery would contribute independently or synergistically to ventilator-induced lung injury (VILI). Methods Under general anesthesia and tracheal intubation, healthy female C57BL/6 N mice (9 weeks old) were randomly ventilated for 2 h by standard (7 ml/kg) or high (14 ml/kg) tidal volume at positive end-expiratory pressure (PEEP) of 2 cmH2O, with room air, 50% O2 (moderate hyperoxia), or 100% O2 (severe hyperoxia); respectively. Mice were sacrificed 4 h after mechanical ventilation, and lung tissues were collected for experimental assessments on lung injury. Results Compared with the healthy control, severe hyperoxia ventilation by either standard or high tidal volume resulted in significantly higher wet-to-dry lung weight ratio and higher levels of IL-1β and 8-OHdG in the lungs. However, moderate hyperoxia ventilation, even by high tidal volume did not significantly increase the levels of IL-1β and 8-OHdG in the lungs. Western blot analysis showed that the expression of RhoA, ROCK1, MLC2, and p-MLC2 was not significantly induced in the ventilated lungs, even by high tidal volume at 2 cmH2O PEEP. Conclusion Severe hyperoxia ventilation causes inflammatory response and oxidative damage in mechanically ventilated lungs, while high tidal volume ventilation at a reasonable PEEP possibly does not cause VILI.https://doi.org/10.1186/s12890-023-02626-xMechanical ventilationTidal volumeOxidative damageInflammatory responseMechanotransduction |
| spellingShingle | Yong Xu Yu Li Da Zhai Chen Yan Jingyan Liang Taiga Ichinomiya Tetsuya Hara Chiaki Inadomi Tao-Sheng Li Hyperoxia but not high tidal volume contributes to ventilator-induced lung injury in healthy mice BMC Pulmonary Medicine Mechanical ventilation Tidal volume Oxidative damage Inflammatory response Mechanotransduction |
| title | Hyperoxia but not high tidal volume contributes to ventilator-induced lung injury in healthy mice |
| title_full | Hyperoxia but not high tidal volume contributes to ventilator-induced lung injury in healthy mice |
| title_fullStr | Hyperoxia but not high tidal volume contributes to ventilator-induced lung injury in healthy mice |
| title_full_unstemmed | Hyperoxia but not high tidal volume contributes to ventilator-induced lung injury in healthy mice |
| title_short | Hyperoxia but not high tidal volume contributes to ventilator-induced lung injury in healthy mice |
| title_sort | hyperoxia but not high tidal volume contributes to ventilator induced lung injury in healthy mice |
| topic | Mechanical ventilation Tidal volume Oxidative damage Inflammatory response Mechanotransduction |
| url | https://doi.org/10.1186/s12890-023-02626-x |
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