Complementary Food Ingredients Alter Infant Gut Microbiome Composition and Metabolism In Vitro
We examined the prebiotic potential of 32 food ingredients on the developing infant microbiome using an in vitro gastroileal digestion and colonic fermentation model. There were significant changes in the concentrations of short-chain fatty-acid metabolites, confirming the potential of the tested in...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-10-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/9/10/2089 |
| _version_ | 1797513791120867328 |
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| author | Shanthi G. Parkar Doug I. Rosendale Halina M. Stoklosinski Carel M. H. Jobsis Duncan I. Hedderley Pramod Gopal |
| author_facet | Shanthi G. Parkar Doug I. Rosendale Halina M. Stoklosinski Carel M. H. Jobsis Duncan I. Hedderley Pramod Gopal |
| author_sort | Shanthi G. Parkar |
| collection | DOAJ |
| description | We examined the prebiotic potential of 32 food ingredients on the developing infant microbiome using an in vitro gastroileal digestion and colonic fermentation model. There were significant changes in the concentrations of short-chain fatty-acid metabolites, confirming the potential of the tested ingredients to stimulate bacterial metabolism. The 16S rRNA gene sequencing for a subset of the ingredients revealed significant increases in the relative abundances of the lactate- and acetate-producing Bifidobacteriaceae, Enterococcaceae, and Lactobacillaceae, and lactate- and acetate-utilizing Prevotellaceae, Lachnospiraceae, and Veillonellaceae. Selective changes in specific bacterial groups were observed. Infant whole-milk powder and an oat flour enhanced Bifidobacteriaceae and lactic acid bacteria. A New Zealand-origin spinach powder enhanced Prevotellaceae and Lachnospiraceae, while fruit and vegetable powders increased a mixed consortium of beneficial gut microbiota. All food ingredients demonstrated a consistent decrease in <i>Clostridium perfringens</i>, with this organism being increased in the carbohydrate-free water control. While further studies are required, this study demonstrates that the selected food ingredients can modulate the infant gut microbiome composition and metabolism in vitro. This approach provides an opportunity to design nutrient-rich complementary foods that fulfil infants’ growth needs and support the maturation of the infant gut microbiome. |
| first_indexed | 2024-03-10T06:22:30Z |
| format | Article |
| id | doaj.art-d9f4acbc6f244c3d8e447438ec580c37 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T06:22:30Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-d9f4acbc6f244c3d8e447438ec580c372023-11-22T19:14:06ZengMDPI AGMicroorganisms2076-26072021-10-01910208910.3390/microorganisms9102089Complementary Food Ingredients Alter Infant Gut Microbiome Composition and Metabolism In VitroShanthi G. Parkar0Doug I. Rosendale1Halina M. Stoklosinski2Carel M. H. Jobsis3Duncan I. Hedderley4Pramod Gopal5The New Zealand Institute for Plant and Food Research Limited, Private Bag 11600, Palmerston North 4442, New ZealandThe New Zealand Institute for Plant and Food Research Limited, Private Bag 11600, Palmerston North 4442, New ZealandThe New Zealand Institute for Plant and Food Research Limited, Private Bag 11600, Palmerston North 4442, New ZealandThe New Zealand Institute for Plant and Food Research Limited, Private Bag 11600, Palmerston North 4442, New ZealandThe New Zealand Institute for Plant and Food Research Limited, Private Bag 11600, Palmerston North 4442, New ZealandThe New Zealand Institute for Plant and Food Research Limited, Private Bag 11600, Palmerston North 4442, New ZealandWe examined the prebiotic potential of 32 food ingredients on the developing infant microbiome using an in vitro gastroileal digestion and colonic fermentation model. There were significant changes in the concentrations of short-chain fatty-acid metabolites, confirming the potential of the tested ingredients to stimulate bacterial metabolism. The 16S rRNA gene sequencing for a subset of the ingredients revealed significant increases in the relative abundances of the lactate- and acetate-producing Bifidobacteriaceae, Enterococcaceae, and Lactobacillaceae, and lactate- and acetate-utilizing Prevotellaceae, Lachnospiraceae, and Veillonellaceae. Selective changes in specific bacterial groups were observed. Infant whole-milk powder and an oat flour enhanced Bifidobacteriaceae and lactic acid bacteria. A New Zealand-origin spinach powder enhanced Prevotellaceae and Lachnospiraceae, while fruit and vegetable powders increased a mixed consortium of beneficial gut microbiota. All food ingredients demonstrated a consistent decrease in <i>Clostridium perfringens</i>, with this organism being increased in the carbohydrate-free water control. While further studies are required, this study demonstrates that the selected food ingredients can modulate the infant gut microbiome composition and metabolism in vitro. This approach provides an opportunity to design nutrient-rich complementary foods that fulfil infants’ growth needs and support the maturation of the infant gut microbiome.https://www.mdpi.com/2076-2607/9/10/2089infant complementary foodsbaby foodsgut microbiomeinfant complementary feedinginfant solid foodsshort-chain fatty acids |
| spellingShingle | Shanthi G. Parkar Doug I. Rosendale Halina M. Stoklosinski Carel M. H. Jobsis Duncan I. Hedderley Pramod Gopal Complementary Food Ingredients Alter Infant Gut Microbiome Composition and Metabolism In Vitro Microorganisms infant complementary foods baby foods gut microbiome infant complementary feeding infant solid foods short-chain fatty acids |
| title | Complementary Food Ingredients Alter Infant Gut Microbiome Composition and Metabolism In Vitro |
| title_full | Complementary Food Ingredients Alter Infant Gut Microbiome Composition and Metabolism In Vitro |
| title_fullStr | Complementary Food Ingredients Alter Infant Gut Microbiome Composition and Metabolism In Vitro |
| title_full_unstemmed | Complementary Food Ingredients Alter Infant Gut Microbiome Composition and Metabolism In Vitro |
| title_short | Complementary Food Ingredients Alter Infant Gut Microbiome Composition and Metabolism In Vitro |
| title_sort | complementary food ingredients alter infant gut microbiome composition and metabolism in vitro |
| topic | infant complementary foods baby foods gut microbiome infant complementary feeding infant solid foods short-chain fatty acids |
| url | https://www.mdpi.com/2076-2607/9/10/2089 |
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