A Generic Procedure for the Isolation of pH- and Magnesium-Responsive Chicken scFvs for Downstream Purification of Human Antibodies

A Generic Procedure for the Isolation of pH- and Magnesium-Responsive Chicken scFvs for Downstream Purification of Human Antibodies

Affinity chromatography provides an excellent platform for protein purification, which is a key step in the large scale downstream processing of therapeutic monoclonal antibodies (Mabs). Protein A chromatography constitutes the gold standard for Mab purification. However, the required acidic conditi...

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Bibliographic Details
Main Authors: Steffen C. Hinz, Adrian Elter, Oliver Rammo, Achim Schwämmle, Ataurehman Ali, Stefan Zielonka, Thomas Herget, Harald Kolmar
Format: Article
Language:English
Published: Frontiers Media S.A. 2020-06-01
Series:Frontiers in Bioengineering and Biotechnology
Subjects:
affinity chromatography
yeast display
protein purification
immune library
chicken antibody
single chain fragment variable
Online Access:https://www.frontiersin.org/article/10.3389/fbioe.2020.00688/full

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https://www.frontiersin.org/article/10.3389/fbioe.2020.00688/full

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