A Generic Procedure for the Isolation of pH- and Magnesium-Responsive Chicken scFvs for Downstream Purification of Human Antibodies
Affinity chromatography provides an excellent platform for protein purification, which is a key step in the large scale downstream processing of therapeutic monoclonal antibodies (Mabs). Protein A chromatography constitutes the gold standard for Mab purification. However, the required acidic conditi...
Main Authors: | Steffen C. Hinz, Adrian Elter, Oliver Rammo, Achim Schwämmle, Ataurehman Ali, Stefan Zielonka, Thomas Herget, Harald Kolmar |
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Format: | Article |
Language: | English |
Published: |
Frontiers Media S.A.
2020-06-01
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Series: | Frontiers in Bioengineering and Biotechnology |
Subjects: | |
Online Access: | https://www.frontiersin.org/article/10.3389/fbioe.2020.00688/full |
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