BRAF-activated WT1 contributes to cancer growth and regulates autophagy and apoptosis in papillary thyroid carcinoma
Abstract Background Papillary thyroid carcinoma (PTC) is one of most prevalent malignant endocrine neoplasms, and it is associated with a high frequency of BRAF gene mutations, which lead to lymphatic metastasis and distant metastasis that promote tumor progression. The molecular mechanism of PTC an...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2022-02-01
|
| Series: | Journal of Translational Medicine |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12967-022-03260-7 |
| _version_ | 1818500874130948096 |
|---|---|
| author | Xing Chen Shan Lin Ying Lin Songsong Wu Minling Zhuo Ailong Zhang Junjie Zheng Zhenhui You |
| author_facet | Xing Chen Shan Lin Ying Lin Songsong Wu Minling Zhuo Ailong Zhang Junjie Zheng Zhenhui You |
| author_sort | Xing Chen |
| collection | DOAJ |
| description | Abstract Background Papillary thyroid carcinoma (PTC) is one of most prevalent malignant endocrine neoplasms, and it is associated with a high frequency of BRAF gene mutations, which lead to lymphatic metastasis and distant metastasis that promote tumor progression. The molecular mechanism of PTC and the role of BRAF mutation in PTC progression and development need to be further elucidated. Methods In this study, a comprehensive bioinformatics analysis was performed to identify the differentially expressed genes and signaling pathways in thyroid cancer patients carrying mutant BRAF. Then, we confirmed the prognostic role of WT1 in thyroid cancer patients. Immunohistochemistry was performed to measure the expression profile of WT1 in PTC tissue. Lentivirus shWT1 was transfected into BRAFV600E (mutant) PTC cells to stably inhibit WT1 expression. CCK-8, EdU, immunofluorescence, colony formation, cell migration, cell wound healing, apoptosis and autophagy assays were performed to assess the biological functions of WT1 in BRAFV600E PTC cells. RNA sequencing, immunohistochemistry and immunoblotting were performed to explore the molecular mechanism of WT1 in BRAFV600E PTC cells. Results The results confirmed that “epithelial cell proliferation”, “apoptosis” and “selective autophagy” were closely associated with this BRAF mutant in these thyroid cancer patients. Knocking down BRAF-activated WT1 effectively inhibited the proliferation and migration of BRAFV600E PTC cells. Silencing WT1 significantly inhibited autophagy and promoted the apoptosis of BRAFV600E PTC cells. Mechanistic investigations showed that silencing WT1 expression remarkably suppressed the AKT/mTOR and ERK/P65 signaling pathways in BRAFV600E PTC cells. Conclusion All these results indicate that WT1 is a promising prognostic biomarker and facilitates PTC progression and development of cells carrying the BRAFV600E mutation. |
| first_indexed | 2024-12-10T20:48:35Z |
| format | Article |
| id | doaj.art-da097ed3fb024e7bad4955bab67b8e0d |
| institution | Directory Open Access Journal |
| issn | 1479-5876 |
| language | English |
| last_indexed | 2024-12-10T20:48:35Z |
| publishDate | 2022-02-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Translational Medicine |
| spelling | doaj.art-da097ed3fb024e7bad4955bab67b8e0d2022-12-22T01:34:10ZengBMCJournal of Translational Medicine1479-58762022-02-0120111610.1186/s12967-022-03260-7BRAF-activated WT1 contributes to cancer growth and regulates autophagy and apoptosis in papillary thyroid carcinomaXing Chen0Shan Lin1Ying Lin2Songsong Wu3Minling Zhuo4Ailong Zhang5Junjie Zheng6Zhenhui You7Department of General Surgery, Fujian Medical University Provincial Clinical Medical College, Fujian Provincial HospitalFujian Medical UniversityDepartment of Obstetrics and Gynaecology, The Hospital of Changle DistrictDepartment of Ultrasonography, Fujian Medical University Provincial Clinical Medical College, Fujian Provincial HospitalDepartment of Ultrasound, Fujian Medical University Union HospitalDepartment of General Surgery, Fujian Medical University Provincial Clinical Medical College, Fujian Provincial HospitalDepartment of General Surgery, Fujian Medical University Provincial Clinical Medical College, Fujian Provincial HospitalDepartment of General Surgery, Fujian Medical University Provincial Clinical Medical College, Fujian Provincial HospitalAbstract Background Papillary thyroid carcinoma (PTC) is one of most prevalent malignant endocrine neoplasms, and it is associated with a high frequency of BRAF gene mutations, which lead to lymphatic metastasis and distant metastasis that promote tumor progression. The molecular mechanism of PTC and the role of BRAF mutation in PTC progression and development need to be further elucidated. Methods In this study, a comprehensive bioinformatics analysis was performed to identify the differentially expressed genes and signaling pathways in thyroid cancer patients carrying mutant BRAF. Then, we confirmed the prognostic role of WT1 in thyroid cancer patients. Immunohistochemistry was performed to measure the expression profile of WT1 in PTC tissue. Lentivirus shWT1 was transfected into BRAFV600E (mutant) PTC cells to stably inhibit WT1 expression. CCK-8, EdU, immunofluorescence, colony formation, cell migration, cell wound healing, apoptosis and autophagy assays were performed to assess the biological functions of WT1 in BRAFV600E PTC cells. RNA sequencing, immunohistochemistry and immunoblotting were performed to explore the molecular mechanism of WT1 in BRAFV600E PTC cells. Results The results confirmed that “epithelial cell proliferation”, “apoptosis” and “selective autophagy” were closely associated with this BRAF mutant in these thyroid cancer patients. Knocking down BRAF-activated WT1 effectively inhibited the proliferation and migration of BRAFV600E PTC cells. Silencing WT1 significantly inhibited autophagy and promoted the apoptosis of BRAFV600E PTC cells. Mechanistic investigations showed that silencing WT1 expression remarkably suppressed the AKT/mTOR and ERK/P65 signaling pathways in BRAFV600E PTC cells. Conclusion All these results indicate that WT1 is a promising prognostic biomarker and facilitates PTC progression and development of cells carrying the BRAFV600E mutation.https://doi.org/10.1186/s12967-022-03260-7PTCWT1AutophagyApoptosisBRAFV600E |
| spellingShingle | Xing Chen Shan Lin Ying Lin Songsong Wu Minling Zhuo Ailong Zhang Junjie Zheng Zhenhui You BRAF-activated WT1 contributes to cancer growth and regulates autophagy and apoptosis in papillary thyroid carcinoma Journal of Translational Medicine PTC WT1 Autophagy Apoptosis BRAFV600E |
| title | BRAF-activated WT1 contributes to cancer growth and regulates autophagy and apoptosis in papillary thyroid carcinoma |
| title_full | BRAF-activated WT1 contributes to cancer growth and regulates autophagy and apoptosis in papillary thyroid carcinoma |
| title_fullStr | BRAF-activated WT1 contributes to cancer growth and regulates autophagy and apoptosis in papillary thyroid carcinoma |
| title_full_unstemmed | BRAF-activated WT1 contributes to cancer growth and regulates autophagy and apoptosis in papillary thyroid carcinoma |
| title_short | BRAF-activated WT1 contributes to cancer growth and regulates autophagy and apoptosis in papillary thyroid carcinoma |
| title_sort | braf activated wt1 contributes to cancer growth and regulates autophagy and apoptosis in papillary thyroid carcinoma |
| topic | PTC WT1 Autophagy Apoptosis BRAFV600E |
| url | https://doi.org/10.1186/s12967-022-03260-7 |
| work_keys_str_mv | AT xingchen brafactivatedwt1contributestocancergrowthandregulatesautophagyandapoptosisinpapillarythyroidcarcinoma AT shanlin brafactivatedwt1contributestocancergrowthandregulatesautophagyandapoptosisinpapillarythyroidcarcinoma AT yinglin brafactivatedwt1contributestocancergrowthandregulatesautophagyandapoptosisinpapillarythyroidcarcinoma AT songsongwu brafactivatedwt1contributestocancergrowthandregulatesautophagyandapoptosisinpapillarythyroidcarcinoma AT minlingzhuo brafactivatedwt1contributestocancergrowthandregulatesautophagyandapoptosisinpapillarythyroidcarcinoma AT ailongzhang brafactivatedwt1contributestocancergrowthandregulatesautophagyandapoptosisinpapillarythyroidcarcinoma AT junjiezheng brafactivatedwt1contributestocancergrowthandregulatesautophagyandapoptosisinpapillarythyroidcarcinoma AT zhenhuiyou brafactivatedwt1contributestocancergrowthandregulatesautophagyandapoptosisinpapillarythyroidcarcinoma |