A Modified Coupled Enzyme Method for O-linked GlcNAc Transferase Activity Assay

A Modified Coupled Enzyme Method for O-linked GlcNAc Transferase Activity Assay

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<p>Abstract</p> <p>In order to determine the activity of O-linked GlcNAc transferase (OGT), a modified coupled enzyme method was proposed. This method was based on the measurement of uridine 5'-(trihydrogen diphosphate) (UDP), a product generated in transglycosylation reaction...

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Bibliographic Details
Main Authors: Zhang Lianwen, Ren Feifei, Li Jing, Ma Xiaofeng, Wang Peng
Format: Article
Language:English
Published: BMC 2009-12-01
Series:Biological Procedures Online
Subjects:
O-linked GlcNAc transferase (OGT)
coupled enzyme method
MS-glycosylation assay
kinetic study
Online Access:http://www.biologicalproceduresonline.com/content/11/1/9016

Internet

http://www.biologicalproceduresonline.com/content/11/1/9016

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