Spatial distribution of elements during osteoarthritis disease progression using synchrotron X-ray fluorescence microscopy
Abstract The osteochondral interface is a thin layer that connects hyaline cartilage to subchondral bone. Subcellular elemental distribution can be visualised using synchrotron X-ray fluorescence microscopy (SR-XFM) (1 μm). This study aims to determine the relationship between elemental distribution...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-06-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-36911-w |
| _version_ | 1827916993778417664 |
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| author | Xiwei Fan Kah Meng Lee Michael W. M. Jones Daryl Howard Antonia Rujia Sun Ross Crawford Indira Prasadam |
| author_facet | Xiwei Fan Kah Meng Lee Michael W. M. Jones Daryl Howard Antonia Rujia Sun Ross Crawford Indira Prasadam |
| author_sort | Xiwei Fan |
| collection | DOAJ |
| description | Abstract The osteochondral interface is a thin layer that connects hyaline cartilage to subchondral bone. Subcellular elemental distribution can be visualised using synchrotron X-ray fluorescence microscopy (SR-XFM) (1 μm). This study aims to determine the relationship between elemental distribution and osteoarthritis (OA) progression based on disease severity. Using modified Mankin scores, we collected tibia plates from 9 knee OA patients who underwent knee replacement surgery and graded them as intact cartilage (non-OA) or degraded cartilage (OA). We used a tape-assisted system with a silicon nitride sandwich structure to collect fresh-frozen osteochondral sections, and changes in the osteochondral unit were defined using quantified SR-XFM elemental mapping at the Australian synchrotron's XFM beamline. Non-OA osteochondral samples were found to have significantly different zinc (Zn) and calcium (Ca) compositions than OA samples. The tidemark separating noncalcified and calcified cartilage was rich in zinc. Zn levels in OA samples were lower than in non-OA samples (P = 0.0072). In OA samples, the tidemark had less Ca than the calcified cartilage zone and subchondral bone plate (P < 0.0001). The Zn–strontium (Sr) colocalisation index was higher in OA samples than in non-OA samples. The lead, potassium, phosphate, sulphur, and chloride distributions were not significantly different (P > 0.05). In conclusion, SR-XFM analysis revealed spatial elemental distribution at the subcellular level during OA development. |
| first_indexed | 2024-03-13T03:22:39Z |
| format | Article |
| id | doaj.art-da2efccb1d094a94a99bc10572e7d604 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-13T03:22:39Z |
| publishDate | 2023-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-da2efccb1d094a94a99bc10572e7d6042023-06-25T11:16:06ZengNature PortfolioScientific Reports2045-23222023-06-0113111210.1038/s41598-023-36911-wSpatial distribution of elements during osteoarthritis disease progression using synchrotron X-ray fluorescence microscopyXiwei Fan0Kah Meng Lee1Michael W. M. Jones2Daryl Howard3Antonia Rujia Sun4Ross Crawford5Indira Prasadam6Centre for Biomedical Technologies, School of Mechanical, Medical and Process Engineering, Queensland University of TechnologyCentral Analytical Research Facility, Queensland University of TechnologyCentral Analytical Research Facility, Queensland University of TechnologyAustralian SynchrotronCentre for Biomedical Technologies, School of Mechanical, Medical and Process Engineering, Queensland University of TechnologyCentre for Biomedical Technologies, School of Mechanical, Medical and Process Engineering, Queensland University of TechnologyCentre for Biomedical Technologies, School of Mechanical, Medical and Process Engineering, Queensland University of TechnologyAbstract The osteochondral interface is a thin layer that connects hyaline cartilage to subchondral bone. Subcellular elemental distribution can be visualised using synchrotron X-ray fluorescence microscopy (SR-XFM) (1 μm). This study aims to determine the relationship between elemental distribution and osteoarthritis (OA) progression based on disease severity. Using modified Mankin scores, we collected tibia plates from 9 knee OA patients who underwent knee replacement surgery and graded them as intact cartilage (non-OA) or degraded cartilage (OA). We used a tape-assisted system with a silicon nitride sandwich structure to collect fresh-frozen osteochondral sections, and changes in the osteochondral unit were defined using quantified SR-XFM elemental mapping at the Australian synchrotron's XFM beamline. Non-OA osteochondral samples were found to have significantly different zinc (Zn) and calcium (Ca) compositions than OA samples. The tidemark separating noncalcified and calcified cartilage was rich in zinc. Zn levels in OA samples were lower than in non-OA samples (P = 0.0072). In OA samples, the tidemark had less Ca than the calcified cartilage zone and subchondral bone plate (P < 0.0001). The Zn–strontium (Sr) colocalisation index was higher in OA samples than in non-OA samples. The lead, potassium, phosphate, sulphur, and chloride distributions were not significantly different (P > 0.05). In conclusion, SR-XFM analysis revealed spatial elemental distribution at the subcellular level during OA development.https://doi.org/10.1038/s41598-023-36911-w |
| spellingShingle | Xiwei Fan Kah Meng Lee Michael W. M. Jones Daryl Howard Antonia Rujia Sun Ross Crawford Indira Prasadam Spatial distribution of elements during osteoarthritis disease progression using synchrotron X-ray fluorescence microscopy Scientific Reports |
| title | Spatial distribution of elements during osteoarthritis disease progression using synchrotron X-ray fluorescence microscopy |
| title_full | Spatial distribution of elements during osteoarthritis disease progression using synchrotron X-ray fluorescence microscopy |
| title_fullStr | Spatial distribution of elements during osteoarthritis disease progression using synchrotron X-ray fluorescence microscopy |
| title_full_unstemmed | Spatial distribution of elements during osteoarthritis disease progression using synchrotron X-ray fluorescence microscopy |
| title_short | Spatial distribution of elements during osteoarthritis disease progression using synchrotron X-ray fluorescence microscopy |
| title_sort | spatial distribution of elements during osteoarthritis disease progression using synchrotron x ray fluorescence microscopy |
| url | https://doi.org/10.1038/s41598-023-36911-w |
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