SWISS: multiplexed orthogonal genome editing in plants with a Cas9 nickase and engineered CRISPR RNA scaffolds

SWISS: multiplexed orthogonal genome editing in plants with a Cas9 nickase and engineered CRISPR RNA scaffolds

Abstract We describe here a CRISPR simultaneous and wide-editing induced by a single system (SWISS), in which RNA aptamers engineered in crRNA scaffold recruit their cognate binding proteins fused with cytidine deaminase and adenosine deaminase to Cas9 nickase target sites to generate multiplexed ba...

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Bibliographic Details
Main Authors: Chao Li, Yuan Zong, Shuai Jin, Haocheng Zhu, Dexing Lin, Shengnan Li, Jin-Long Qiu, Yanpeng Wang, Caixia Gao
Format: Article
Language:English
Published: BMC 2020-06-01
Series:Genome Biology
Subjects:
Multiplexed orthogonal genome editing
Cas9 nickase
CBE
ABE
Indels
RNA scaffolds
Online Access:http://link.springer.com/article/10.1186/s13059-020-02051-x

Internet

http://link.springer.com/article/10.1186/s13059-020-02051-x

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