SWISS: multiplexed orthogonal genome editing in plants with a Cas9 nickase and engineered CRISPR RNA scaffolds
Abstract We describe here a CRISPR simultaneous and wide-editing induced by a single system (SWISS), in which RNA aptamers engineered in crRNA scaffold recruit their cognate binding proteins fused with cytidine deaminase and adenosine deaminase to Cas9 nickase target sites to generate multiplexed ba...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2020-06-01
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| Series: | Genome Biology |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13059-020-02051-x |
| _version_ | 1819012354336096256 |
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| author | Chao Li Yuan Zong Shuai Jin Haocheng Zhu Dexing Lin Shengnan Li Jin-Long Qiu Yanpeng Wang Caixia Gao |
| author_facet | Chao Li Yuan Zong Shuai Jin Haocheng Zhu Dexing Lin Shengnan Li Jin-Long Qiu Yanpeng Wang Caixia Gao |
| author_sort | Chao Li |
| collection | DOAJ |
| description | Abstract We describe here a CRISPR simultaneous and wide-editing induced by a single system (SWISS), in which RNA aptamers engineered in crRNA scaffold recruit their cognate binding proteins fused with cytidine deaminase and adenosine deaminase to Cas9 nickase target sites to generate multiplexed base editing. By using paired sgRNAs, SWISS can produce insertions/deletions in addition to base editing. Rice mutants are generated using the SWISS system with efficiencies of cytosine conversion of 25.5%, adenine conversion of 16.4%, indels of 52.7%, and simultaneous triple mutations of 7.3%. The SWISS system provides a powerful tool for multi-functional genome editing in plants. |
| first_indexed | 2024-12-21T01:42:43Z |
| format | Article |
| id | doaj.art-da664272e5b441639ec4000dd23f2a75 |
| institution | Directory Open Access Journal |
| issn | 1474-760X |
| language | English |
| last_indexed | 2024-12-21T01:42:43Z |
| publishDate | 2020-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Genome Biology |
| spelling | doaj.art-da664272e5b441639ec4000dd23f2a752022-12-21T19:20:07ZengBMCGenome Biology1474-760X2020-06-0121111510.1186/s13059-020-02051-xSWISS: multiplexed orthogonal genome editing in plants with a Cas9 nickase and engineered CRISPR RNA scaffoldsChao Li0Yuan Zong1Shuai Jin2Haocheng Zhu3Dexing Lin4Shengnan Li5Jin-Long Qiu6Yanpeng Wang7Caixia Gao8State Key Laboratory of Plant Cell and Chromosome Engineering, Center for Genome Editing, Institute of Genetics and Developmental Biology, Innovation Academy for Seed Design, Chinese Academy of SciencesState Key Laboratory of Plant Cell and Chromosome Engineering, Center for Genome Editing, Institute of Genetics and Developmental Biology, Innovation Academy for Seed Design, Chinese Academy of SciencesState Key Laboratory of Plant Cell and Chromosome Engineering, Center for Genome Editing, Institute of Genetics and Developmental Biology, Innovation Academy for Seed Design, Chinese Academy of SciencesState Key Laboratory of Plant Cell and Chromosome Engineering, Center for Genome Editing, Institute of Genetics and Developmental Biology, Innovation Academy for Seed Design, Chinese Academy of SciencesState Key Laboratory of Plant Cell and Chromosome Engineering, Center for Genome Editing, Institute of Genetics and Developmental Biology, Innovation Academy for Seed Design, Chinese Academy of SciencesState Key Laboratory of Plant Genomics, Institute of Microbiology, Innovation Academy for Seed Design, Chinese Academy of SciencesCollege of Advanced Agricultural Sciences, University of Chinese Academy of SciencesState Key Laboratory of Plant Cell and Chromosome Engineering, Center for Genome Editing, Institute of Genetics and Developmental Biology, Innovation Academy for Seed Design, Chinese Academy of SciencesState Key Laboratory of Plant Cell and Chromosome Engineering, Center for Genome Editing, Institute of Genetics and Developmental Biology, Innovation Academy for Seed Design, Chinese Academy of SciencesAbstract We describe here a CRISPR simultaneous and wide-editing induced by a single system (SWISS), in which RNA aptamers engineered in crRNA scaffold recruit their cognate binding proteins fused with cytidine deaminase and adenosine deaminase to Cas9 nickase target sites to generate multiplexed base editing. By using paired sgRNAs, SWISS can produce insertions/deletions in addition to base editing. Rice mutants are generated using the SWISS system with efficiencies of cytosine conversion of 25.5%, adenine conversion of 16.4%, indels of 52.7%, and simultaneous triple mutations of 7.3%. The SWISS system provides a powerful tool for multi-functional genome editing in plants.http://link.springer.com/article/10.1186/s13059-020-02051-xMultiplexed orthogonal genome editingCas9 nickaseCBEABEIndelsRNA scaffolds |
| spellingShingle | Chao Li Yuan Zong Shuai Jin Haocheng Zhu Dexing Lin Shengnan Li Jin-Long Qiu Yanpeng Wang Caixia Gao SWISS: multiplexed orthogonal genome editing in plants with a Cas9 nickase and engineered CRISPR RNA scaffolds Genome Biology Multiplexed orthogonal genome editing Cas9 nickase CBE ABE Indels RNA scaffolds |
| title | SWISS: multiplexed orthogonal genome editing in plants with a Cas9 nickase and engineered CRISPR RNA scaffolds |
| title_full | SWISS: multiplexed orthogonal genome editing in plants with a Cas9 nickase and engineered CRISPR RNA scaffolds |
| title_fullStr | SWISS: multiplexed orthogonal genome editing in plants with a Cas9 nickase and engineered CRISPR RNA scaffolds |
| title_full_unstemmed | SWISS: multiplexed orthogonal genome editing in plants with a Cas9 nickase and engineered CRISPR RNA scaffolds |
| title_short | SWISS: multiplexed orthogonal genome editing in plants with a Cas9 nickase and engineered CRISPR RNA scaffolds |
| title_sort | swiss multiplexed orthogonal genome editing in plants with a cas9 nickase and engineered crispr rna scaffolds |
| topic | Multiplexed orthogonal genome editing Cas9 nickase CBE ABE Indels RNA scaffolds |
| url | http://link.springer.com/article/10.1186/s13059-020-02051-x |
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