The bioactive peptide VLATSGPG regulates the abnormal lipid accumulation and inflammation induced by free fatty acids in HepG2 cells via the PERK signaling pathway
VLATSGPG (VLA) was a DPP-IV inhibitory peptide isolated from salmon (Salmo Salar) skin. Here, we demonstrated that VLA inhibited the activation of PERK through the AKT signaling pathway. Furthermore, we investigated the effect of VLA on free fatty acid (FFA)-induced lipid accumulation disorder and i...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-05-01
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| Series: | Journal of Functional Foods |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464623001159 |
| _version_ | 1797837127402127360 |
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| author | Ritian Jin Jude Juventus Aweya Rong Lin Wuyin Weng Jiaqi Shang Dangfeng Wang Yiling Fan Shen Yang |
| author_facet | Ritian Jin Jude Juventus Aweya Rong Lin Wuyin Weng Jiaqi Shang Dangfeng Wang Yiling Fan Shen Yang |
| author_sort | Ritian Jin |
| collection | DOAJ |
| description | VLATSGPG (VLA) was a DPP-IV inhibitory peptide isolated from salmon (Salmo Salar) skin. Here, we demonstrated that VLA inhibited the activation of PERK through the AKT signaling pathway. Furthermore, we investigated the effect of VLA on free fatty acid (FFA)-induced lipid accumulation disorder and inflammation in HepG2 cells, elucidating the potential regulatory mechanism in this nonalcoholic fatty liver disease cell model. VLA reduced the mRNA expression of adipogenic factors FAS, ACC, and SCD-1 through the PERK/eIF2α pathway and the level of apolipoprotein B-100 secretion. These may contribute to the reducing FFA-induced lipid accumulation in cells and reducing intracellular lipid levels. In addition, VLA increased IκBα mRNA expression via the PERK/IκBα pathway, inhibited the NF-κB p65 activation, and thus inhibited cell inflammation. |
| first_indexed | 2024-04-09T15:20:55Z |
| format | Article |
| id | doaj.art-da66c9431dad4e6dbc80b12288a03217 |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-04-09T15:20:55Z |
| publishDate | 2023-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-da66c9431dad4e6dbc80b12288a032172023-04-29T14:48:23ZengElsevierJournal of Functional Foods1756-46462023-05-01104105515The bioactive peptide VLATSGPG regulates the abnormal lipid accumulation and inflammation induced by free fatty acids in HepG2 cells via the PERK signaling pathwayRitian Jin0Jude Juventus Aweya1Rong Lin2Wuyin Weng3Jiaqi Shang4Dangfeng Wang5Yiling Fan6Shen Yang7College of Ocean Food and Biological Engineering, Jimei University, Xiamen 361021, China; Fujian Provincial Key Laboratory of Food Microbiology and Enzyme Engineering, Jimei University, 361021, ChinaCollege of Ocean Food and Biological Engineering, Jimei University, Xiamen 361021, China; Fujian Provincial Key Laboratory of Food Microbiology and Enzyme Engineering, Jimei University, 361021, ChinaCollege of Ocean Food and Biological Engineering, Jimei University, Xiamen 361021, China; Fujian Provincial Key Laboratory of Food Microbiology and Enzyme Engineering, Jimei University, 361021, ChinaCollege of Ocean Food and Biological Engineering, Jimei University, Xiamen 361021, ChinaCollege of Biological and Agricultural Engineering, Jilin University, Changchun 130022, ChinaCollege of Food Science and Technology, Jiangnan University, Wuxi 214122, ChinaCollege of Ocean Food and Biological Engineering, Jimei University, Xiamen 361021, ChinaCollege of Ocean Food and Biological Engineering, Jimei University, Xiamen 361021, China; Fujian Provincial Key Laboratory of Food Microbiology and Enzyme Engineering, Jimei University, 361021, China; Corresponding author at: College of Ocean Food and Biological Engineering, Jimei University, 43 Yindou Road, Xiamen 361021, China.VLATSGPG (VLA) was a DPP-IV inhibitory peptide isolated from salmon (Salmo Salar) skin. Here, we demonstrated that VLA inhibited the activation of PERK through the AKT signaling pathway. Furthermore, we investigated the effect of VLA on free fatty acid (FFA)-induced lipid accumulation disorder and inflammation in HepG2 cells, elucidating the potential regulatory mechanism in this nonalcoholic fatty liver disease cell model. VLA reduced the mRNA expression of adipogenic factors FAS, ACC, and SCD-1 through the PERK/eIF2α pathway and the level of apolipoprotein B-100 secretion. These may contribute to the reducing FFA-induced lipid accumulation in cells and reducing intracellular lipid levels. In addition, VLA increased IκBα mRNA expression via the PERK/IκBα pathway, inhibited the NF-κB p65 activation, and thus inhibited cell inflammation.http://www.sciencedirect.com/science/article/pii/S1756464623001159DPP-IV inhibitory peptideFree fatty acidsNAFLDPERKHepG2 |
| spellingShingle | Ritian Jin Jude Juventus Aweya Rong Lin Wuyin Weng Jiaqi Shang Dangfeng Wang Yiling Fan Shen Yang The bioactive peptide VLATSGPG regulates the abnormal lipid accumulation and inflammation induced by free fatty acids in HepG2 cells via the PERK signaling pathway Journal of Functional Foods DPP-IV inhibitory peptide Free fatty acids NAFLD PERK HepG2 |
| title | The bioactive peptide VLATSGPG regulates the abnormal lipid accumulation and inflammation induced by free fatty acids in HepG2 cells via the PERK signaling pathway |
| title_full | The bioactive peptide VLATSGPG regulates the abnormal lipid accumulation and inflammation induced by free fatty acids in HepG2 cells via the PERK signaling pathway |
| title_fullStr | The bioactive peptide VLATSGPG regulates the abnormal lipid accumulation and inflammation induced by free fatty acids in HepG2 cells via the PERK signaling pathway |
| title_full_unstemmed | The bioactive peptide VLATSGPG regulates the abnormal lipid accumulation and inflammation induced by free fatty acids in HepG2 cells via the PERK signaling pathway |
| title_short | The bioactive peptide VLATSGPG regulates the abnormal lipid accumulation and inflammation induced by free fatty acids in HepG2 cells via the PERK signaling pathway |
| title_sort | bioactive peptide vlatsgpg regulates the abnormal lipid accumulation and inflammation induced by free fatty acids in hepg2 cells via the perk signaling pathway |
| topic | DPP-IV inhibitory peptide Free fatty acids NAFLD PERK HepG2 |
| url | http://www.sciencedirect.com/science/article/pii/S1756464623001159 |
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