Antimicrobial Resistance Risk Assessment of <i>Vibrio parahaemolyticus</i> Isolated from Farmed Green Mussels in Singapore
<i>Vibrio parahaemolyticus</i>, commonly found in seafood products, is responsible for gastroenteritis resulting from the consumption of undercooked seafood. Hence, there is a need to characterize and quantify the risk involved from this pathogen. However, there has been no study reporti...
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MDPI AG
2023-06-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/11/6/1498 |
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| author | Hong Ming Glendon Ong Yang Zhong Chengcheng Hu Kar Hui Ong Wei Ching Khor Joergen Schlundt Kyaw Thu Aung |
| author_facet | Hong Ming Glendon Ong Yang Zhong Chengcheng Hu Kar Hui Ong Wei Ching Khor Joergen Schlundt Kyaw Thu Aung |
| author_sort | Hong Ming Glendon Ong |
| collection | DOAJ |
| description | <i>Vibrio parahaemolyticus</i>, commonly found in seafood products, is responsible for gastroenteritis resulting from the consumption of undercooked seafood. Hence, there is a need to characterize and quantify the risk involved from this pathogen. However, there has been no study reporting the quantification of hemolytic antimicrobial-resistant (AMR) <i>Vibrio parahaemolyticus</i> in locally farmed shellfish in Singapore. In this study, ampicillin, penicillin G, tetracycline resistant, and non-AMR hemolytic <i>V. parahaemolyticus</i> were surveyed and quantified in green mussel samples from different premises in the food chain (farm and retail). The occurrence data showed that 31/45 (68.9%) of farmed green mussel samples, 6/6 (100%) farm water samples, and 41/45 (91.1%) retail shellfish samples detected the presence of hemolytic <i>V. parahaemolyticus. V. parahaemolyticus</i> counts ranged from 1.6–5.9 Log CFU/g in the retail shellfish samples and 1.0–2.9 Log CFU/g in the farm water samples. AMR risk assessments (ARRA), specifically for ampicillin, penicillin G, tetracycline, and hemolytic (non-AMR) scenarios were conducted for the full farm-to-home and partial retail-to-home chains. The hemolytic ARRA scenario estimated an average probability of illness of 5.7 × 10<sup>−3</sup> and 1.2 × 10<sup>−2</sup> per serving for the full and partial chains, respectively, translating to 165 and 355 annual cases per total population or 2.9 and 6.2 cases per 100,000 population, respectively. The average probability of illness per year ratios for the three ARRAs to the hemolytic ARRA were 0.82, 0.81, and 0.47 (ampicillin, penicillin G, and tetracycline, respectively) for the full chain and 0.54, 0.39, and 0.09 (ampicillin, penicillin G, and tetracycline, respectively) for the partial chain. The sensitivity analysis showed that the overall cooking effect, initial concentrations of pathogenic <i>V. parahaemolyticus</i>, and harvest duration and harvest temperature were key variables influencing the risk estimates in all of the modelled ARRAs. The study findings can be used by relevant stakeholders to make informed decisions for risk management that improve food safety. |
| first_indexed | 2024-03-11T02:08:43Z |
| format | Article |
| id | doaj.art-da748c2b366b4da2987a98e9a3362d41 |
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| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-11T02:08:43Z |
| publishDate | 2023-06-01 |
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| spelling | doaj.art-da748c2b366b4da2987a98e9a3362d412023-11-18T11:42:43ZengMDPI AGMicroorganisms2076-26072023-06-01116149810.3390/microorganisms11061498Antimicrobial Resistance Risk Assessment of <i>Vibrio parahaemolyticus</i> Isolated from Farmed Green Mussels in SingaporeHong Ming Glendon Ong0Yang Zhong1Chengcheng Hu2Kar Hui Ong3Wei Ching Khor4Joergen Schlundt5Kyaw Thu Aung6School of Chemistry, Chemical Engineering and Biotechnology, Nanyang Technological University, Block N1.2, B3-15, 62 Nanyang Drive, Singapore 637459, SingaporeDepartment of Clinical Translational Research, Singapore General Hospital, Academia, 20 College Road, Singapore 169856, SingaporeSingapore Institute of Manufacturing Technology, 08-04, Innovis, 2 Fusionopolis Way, Singapore 138634, SingaporeNational Centre for Food Science, Singapore Food Agency, 7 International Business Park, Techquest, Singapore 609919, SingaporeNational Centre for Food Science, Singapore Food Agency, 7 International Business Park, Techquest, Singapore 609919, SingaporeSchlundt Consult, 3250 Gilleleje, DenmarkNational Centre for Food Science, Singapore Food Agency, 7 International Business Park, Techquest, Singapore 609919, Singapore<i>Vibrio parahaemolyticus</i>, commonly found in seafood products, is responsible for gastroenteritis resulting from the consumption of undercooked seafood. Hence, there is a need to characterize and quantify the risk involved from this pathogen. However, there has been no study reporting the quantification of hemolytic antimicrobial-resistant (AMR) <i>Vibrio parahaemolyticus</i> in locally farmed shellfish in Singapore. In this study, ampicillin, penicillin G, tetracycline resistant, and non-AMR hemolytic <i>V. parahaemolyticus</i> were surveyed and quantified in green mussel samples from different premises in the food chain (farm and retail). The occurrence data showed that 31/45 (68.9%) of farmed green mussel samples, 6/6 (100%) farm water samples, and 41/45 (91.1%) retail shellfish samples detected the presence of hemolytic <i>V. parahaemolyticus. V. parahaemolyticus</i> counts ranged from 1.6–5.9 Log CFU/g in the retail shellfish samples and 1.0–2.9 Log CFU/g in the farm water samples. AMR risk assessments (ARRA), specifically for ampicillin, penicillin G, tetracycline, and hemolytic (non-AMR) scenarios were conducted for the full farm-to-home and partial retail-to-home chains. The hemolytic ARRA scenario estimated an average probability of illness of 5.7 × 10<sup>−3</sup> and 1.2 × 10<sup>−2</sup> per serving for the full and partial chains, respectively, translating to 165 and 355 annual cases per total population or 2.9 and 6.2 cases per 100,000 population, respectively. The average probability of illness per year ratios for the three ARRAs to the hemolytic ARRA were 0.82, 0.81, and 0.47 (ampicillin, penicillin G, and tetracycline, respectively) for the full chain and 0.54, 0.39, and 0.09 (ampicillin, penicillin G, and tetracycline, respectively) for the partial chain. The sensitivity analysis showed that the overall cooking effect, initial concentrations of pathogenic <i>V. parahaemolyticus</i>, and harvest duration and harvest temperature were key variables influencing the risk estimates in all of the modelled ARRAs. The study findings can be used by relevant stakeholders to make informed decisions for risk management that improve food safety.https://www.mdpi.com/2076-2607/11/6/1498antimicrobial resistance risk assessmentemolytic@Riskfarm-to-homeretail-to-homesensitivity analysis |
| spellingShingle | Hong Ming Glendon Ong Yang Zhong Chengcheng Hu Kar Hui Ong Wei Ching Khor Joergen Schlundt Kyaw Thu Aung Antimicrobial Resistance Risk Assessment of <i>Vibrio parahaemolyticus</i> Isolated from Farmed Green Mussels in Singapore Microorganisms antimicrobial resistance risk assessment emolytic @Risk farm-to-home retail-to-home sensitivity analysis |
| title | Antimicrobial Resistance Risk Assessment of <i>Vibrio parahaemolyticus</i> Isolated from Farmed Green Mussels in Singapore |
| title_full | Antimicrobial Resistance Risk Assessment of <i>Vibrio parahaemolyticus</i> Isolated from Farmed Green Mussels in Singapore |
| title_fullStr | Antimicrobial Resistance Risk Assessment of <i>Vibrio parahaemolyticus</i> Isolated from Farmed Green Mussels in Singapore |
| title_full_unstemmed | Antimicrobial Resistance Risk Assessment of <i>Vibrio parahaemolyticus</i> Isolated from Farmed Green Mussels in Singapore |
| title_short | Antimicrobial Resistance Risk Assessment of <i>Vibrio parahaemolyticus</i> Isolated from Farmed Green Mussels in Singapore |
| title_sort | antimicrobial resistance risk assessment of i vibrio parahaemolyticus i isolated from farmed green mussels in singapore |
| topic | antimicrobial resistance risk assessment emolytic @Risk farm-to-home retail-to-home sensitivity analysis |
| url | https://www.mdpi.com/2076-2607/11/6/1498 |
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