Factors influencing cell differentiation and expressions of cytochrome P450s and transporters in Caco-2 cells
Absorption, transportation, and first-pass metabolism of xenobiotics, drugs and nutrients taken orally occurs in the intestine, which is a major organ expressing cytochrome P450 enzymes (CYP) and transporters. Caco-2 is a human colon carcinoma cell line widely employed as a model for xenobiotic ab...
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| Format: | Article |
| Language: | English |
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Prince of Songkla University
2021-06-01
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| Series: | Songklanakarin Journal of Science and Technology (SJST) |
| Subjects: | |
| Online Access: | https://rdo.psu.ac.th/sjstweb/journal/43-3/26.pdf |
| _version_ | 1818657243575353344 |
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| author | Nathaphon Kuncharoenwirat Waranya Chatuphonprasert Kanokwan Jarukamjorn |
| author_facet | Nathaphon Kuncharoenwirat Waranya Chatuphonprasert Kanokwan Jarukamjorn |
| author_sort | Nathaphon Kuncharoenwirat |
| collection | DOAJ |
| description | Absorption, transportation, and first-pass metabolism of xenobiotics, drugs and nutrients taken orally occurs in the
intestine, which is a major organ expressing cytochrome P450 enzymes (CYP) and transporters. Caco-2 is a human colon
carcinoma cell line widely employed as a model for xenobiotic absorption, transportation, and metabolism in the intestinal tract
as it can spontaneously differentiate to become small intestinal enterocytes. Culturing factors influence differentiation of Caco-2
as well as expression of CYPs and drug transporters. Culturing period, medium composition, type of filter membrane inserts, and
the seeding density, account for the major factors affecting cell differentiation and the expression of CYP and transporter.
Therefore, determining the optimal conditions for Caco-2 culturing is of strong concern depending on the aim and experimental
design of the study. |
| first_indexed | 2024-12-17T03:38:23Z |
| format | Article |
| id | doaj.art-da79b8f6bc6442fe886fd0c9b25484f0 |
| institution | Directory Open Access Journal |
| issn | 0125-3395 |
| language | English |
| last_indexed | 2024-12-17T03:38:23Z |
| publishDate | 2021-06-01 |
| publisher | Prince of Songkla University |
| record_format | Article |
| series | Songklanakarin Journal of Science and Technology (SJST) |
| spelling | doaj.art-da79b8f6bc6442fe886fd0c9b25484f02022-12-21T22:05:04ZengPrince of Songkla UniversitySongklanakarin Journal of Science and Technology (SJST)0125-33952021-06-0143380280910.14456/sjst-psu.2021.106Factors influencing cell differentiation and expressions of cytochrome P450s and transporters in Caco-2 cellsNathaphon Kuncharoenwirat0Waranya Chatuphonprasert1Kanokwan Jarukamjorn2Research Group for Pharmaceutical Activities of Natural Products using Pharmaceutical Biotechnology (PANPB), Faculty of Pharmaceutical Sciences, Khon Kaen University, Mueang, Khon Kaen, 40002 ThailandFaculty of Medicine, Mahasarakham University, Mueang, Mahasarakham 44000 ThailandResearch Group for Pharmaceutical Activities of Natural Products using Pharmaceutical Biotechnology (PANPB), Faculty of Pharmaceutical Sciences, Khon Kaen University, Mueang, Khon Kaen, 40002 ThailandAbsorption, transportation, and first-pass metabolism of xenobiotics, drugs and nutrients taken orally occurs in the intestine, which is a major organ expressing cytochrome P450 enzymes (CYP) and transporters. Caco-2 is a human colon carcinoma cell line widely employed as a model for xenobiotic absorption, transportation, and metabolism in the intestinal tract as it can spontaneously differentiate to become small intestinal enterocytes. Culturing factors influence differentiation of Caco-2 as well as expression of CYPs and drug transporters. Culturing period, medium composition, type of filter membrane inserts, and the seeding density, account for the major factors affecting cell differentiation and the expression of CYP and transporter. Therefore, determining the optimal conditions for Caco-2 culturing is of strong concern depending on the aim and experimental design of the study.https://rdo.psu.ac.th/sjstweb/journal/43-3/26.pdfcytochrome p450transportercaco-2differentiationintestinal enterocyte |
| spellingShingle | Nathaphon Kuncharoenwirat Waranya Chatuphonprasert Kanokwan Jarukamjorn Factors influencing cell differentiation and expressions of cytochrome P450s and transporters in Caco-2 cells Songklanakarin Journal of Science and Technology (SJST) cytochrome p450 transporter caco-2 differentiation intestinal enterocyte |
| title | Factors influencing cell differentiation and expressions of cytochrome P450s and transporters in Caco-2 cells |
| title_full | Factors influencing cell differentiation and expressions of cytochrome P450s and transporters in Caco-2 cells |
| title_fullStr | Factors influencing cell differentiation and expressions of cytochrome P450s and transporters in Caco-2 cells |
| title_full_unstemmed | Factors influencing cell differentiation and expressions of cytochrome P450s and transporters in Caco-2 cells |
| title_short | Factors influencing cell differentiation and expressions of cytochrome P450s and transporters in Caco-2 cells |
| title_sort | factors influencing cell differentiation and expressions of cytochrome p450s and transporters in caco 2 cells |
| topic | cytochrome p450 transporter caco-2 differentiation intestinal enterocyte |
| url | https://rdo.psu.ac.th/sjstweb/journal/43-3/26.pdf |
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