Inference of relationships in population data using identity-by-descent and identity-by-state.
It is an assumption of large, population-based datasets that samples are annotated accurately whether they correspond to known relationships or unrelated individuals. These annotations are key for a broad range of genetics applications. While many methods are available to assess relatedness that inv...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2011-09-01
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| Series: | PLoS Genetics |
| Online Access: | http://europepmc.org/articles/PMC3178600?pdf=render |
| _version_ | 1828328862517297152 |
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| author | Eric L Stevens Greg Heckenberg Elisha D O Roberson Joseph D Baugher Thomas J Downey Jonathan Pevsner |
| author_facet | Eric L Stevens Greg Heckenberg Elisha D O Roberson Joseph D Baugher Thomas J Downey Jonathan Pevsner |
| author_sort | Eric L Stevens |
| collection | DOAJ |
| description | It is an assumption of large, population-based datasets that samples are annotated accurately whether they correspond to known relationships or unrelated individuals. These annotations are key for a broad range of genetics applications. While many methods are available to assess relatedness that involve estimates of identity-by-descent (IBD) and/or identity-by-state (IBS) allele-sharing proportions, we developed a novel approach that estimates IBD0, 1, and 2 based on observed IBS within windows. When combined with genome-wide IBS information, it provides an intuitive and practical graphical approach with the capacity to analyze datasets with thousands of samples without prior information about relatedness between individuals or haplotypes. We applied the method to a commonly used Human Variation Panel consisting of 400 nominally unrelated individuals. Surprisingly, we identified identical, parent-child, and full-sibling relationships and reconstructed pedigrees. In two instances non-sibling pairs of individuals in these pedigrees had unexpected IBD2 levels, as well as multiple regions of homozygosity, implying inbreeding. This combined method allowed us to distinguish related individuals from those having atypical heterozygosity rates and determine which individuals were outliers with respect to their designated population. Additionally, it becomes increasingly difficult to identify distant relatedness using genome-wide IBS methods alone. However, our IBD method further identified distant relatedness between individuals within populations, supported by the presence of megabase-scale regions lacking IBS0 across individual chromosomes. We benchmarked our approach against the hidden Markov model of a leading software package (PLINK), showing improved calling of distantly related individuals, and we validated it using a known pedigree from a clinical study. The application of this approach could improve genome-wide association, linkage, heterozygosity, and other population genomics studies that rely on SNP genotype data. |
| first_indexed | 2024-04-13T20:15:53Z |
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| id | doaj.art-da937ee947e642ca8859c0eb29cb325c |
| institution | Directory Open Access Journal |
| issn | 1553-7390 1553-7404 |
| language | English |
| last_indexed | 2024-04-13T20:15:53Z |
| publishDate | 2011-09-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Genetics |
| spelling | doaj.art-da937ee947e642ca8859c0eb29cb325c2022-12-22T02:31:43ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042011-09-0179e100228710.1371/journal.pgen.1002287Inference of relationships in population data using identity-by-descent and identity-by-state.Eric L StevensGreg HeckenbergElisha D O RobersonJoseph D BaugherThomas J DowneyJonathan PevsnerIt is an assumption of large, population-based datasets that samples are annotated accurately whether they correspond to known relationships or unrelated individuals. These annotations are key for a broad range of genetics applications. While many methods are available to assess relatedness that involve estimates of identity-by-descent (IBD) and/or identity-by-state (IBS) allele-sharing proportions, we developed a novel approach that estimates IBD0, 1, and 2 based on observed IBS within windows. When combined with genome-wide IBS information, it provides an intuitive and practical graphical approach with the capacity to analyze datasets with thousands of samples without prior information about relatedness between individuals or haplotypes. We applied the method to a commonly used Human Variation Panel consisting of 400 nominally unrelated individuals. Surprisingly, we identified identical, parent-child, and full-sibling relationships and reconstructed pedigrees. In two instances non-sibling pairs of individuals in these pedigrees had unexpected IBD2 levels, as well as multiple regions of homozygosity, implying inbreeding. This combined method allowed us to distinguish related individuals from those having atypical heterozygosity rates and determine which individuals were outliers with respect to their designated population. Additionally, it becomes increasingly difficult to identify distant relatedness using genome-wide IBS methods alone. However, our IBD method further identified distant relatedness between individuals within populations, supported by the presence of megabase-scale regions lacking IBS0 across individual chromosomes. We benchmarked our approach against the hidden Markov model of a leading software package (PLINK), showing improved calling of distantly related individuals, and we validated it using a known pedigree from a clinical study. The application of this approach could improve genome-wide association, linkage, heterozygosity, and other population genomics studies that rely on SNP genotype data.http://europepmc.org/articles/PMC3178600?pdf=render |
| spellingShingle | Eric L Stevens Greg Heckenberg Elisha D O Roberson Joseph D Baugher Thomas J Downey Jonathan Pevsner Inference of relationships in population data using identity-by-descent and identity-by-state. PLoS Genetics |
| title | Inference of relationships in population data using identity-by-descent and identity-by-state. |
| title_full | Inference of relationships in population data using identity-by-descent and identity-by-state. |
| title_fullStr | Inference of relationships in population data using identity-by-descent and identity-by-state. |
| title_full_unstemmed | Inference of relationships in population data using identity-by-descent and identity-by-state. |
| title_short | Inference of relationships in population data using identity-by-descent and identity-by-state. |
| title_sort | inference of relationships in population data using identity by descent and identity by state |
| url | http://europepmc.org/articles/PMC3178600?pdf=render |
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