The Antioxidant Capacity In Vitro and In Vivo of Polysaccharides From <i>Bergenia emeiensis</i>
Polysaccharides from <i>Bergenia emeiensis</i> (PBE) showed a robust antioxidant ability on scavenging free radicals in vitro. However, the further antioxidant potential in cell level and in vivo was still unknown. Therefore, in this present study, the protective effect of PBE on human c...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-10-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/21/20/7456 |
| _version_ | 1797551444533968896 |
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| author | Chen Zeng Shiling Feng |
| author_facet | Chen Zeng Shiling Feng |
| author_sort | Chen Zeng |
| collection | DOAJ |
| description | Polysaccharides from <i>Bergenia emeiensis</i> (PBE) showed a robust antioxidant ability on scavenging free radicals in vitro. However, the further antioxidant potential in cell level and in vivo was still unknown. Therefore, in this present study, the protective effect of PBE on human cervical carcinoma cell (Hela) cells and <i>Caenorhabditis elegans</i> against oxidative stress was evaluated. The results showed PBE could reduce the reactive oxygen species (ROS) level in Hela cells and promote the mitochondrial membrane potential. Then, the cell apoptosis was reduced. Moreover, PBE could enhance the survival of <i>C. elegans</i> under thermal stress to 13.44%, and significantly reduce the ROS level, which was connected with the overexpression of <i>sod-3</i> and the increased nuclear localization of <i>daf-16</i> transcription factor. Therefore, PBE exhibited a strong antioxidant capacity in the cellular level and for a whole organism. Thus, polysaccharides from <i>B. emeiensis</i> have natural potential to be a safe antioxidant. |
| first_indexed | 2024-03-10T15:46:11Z |
| format | Article |
| id | doaj.art-da9650425886486e90a650f11d24e756 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T15:46:11Z |
| publishDate | 2020-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-da9650425886486e90a650f11d24e7562023-11-20T16:30:46ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-10-012120745610.3390/ijms21207456The Antioxidant Capacity In Vitro and In Vivo of Polysaccharides From <i>Bergenia emeiensis</i>Chen Zeng0Shiling Feng1College of Life Science, Sichuan Agricultural University, Ya’an 625014, ChinaCollege of Life Science, Sichuan Agricultural University, Ya’an 625014, ChinaPolysaccharides from <i>Bergenia emeiensis</i> (PBE) showed a robust antioxidant ability on scavenging free radicals in vitro. However, the further antioxidant potential in cell level and in vivo was still unknown. Therefore, in this present study, the protective effect of PBE on human cervical carcinoma cell (Hela) cells and <i>Caenorhabditis elegans</i> against oxidative stress was evaluated. The results showed PBE could reduce the reactive oxygen species (ROS) level in Hela cells and promote the mitochondrial membrane potential. Then, the cell apoptosis was reduced. Moreover, PBE could enhance the survival of <i>C. elegans</i> under thermal stress to 13.44%, and significantly reduce the ROS level, which was connected with the overexpression of <i>sod-3</i> and the increased nuclear localization of <i>daf-16</i> transcription factor. Therefore, PBE exhibited a strong antioxidant capacity in the cellular level and for a whole organism. Thus, polysaccharides from <i>B. emeiensis</i> have natural potential to be a safe antioxidant.https://www.mdpi.com/1422-0067/21/20/7456<i>Bergenia emeiensis</i>polysaccharidesantioxidant ability<i>C. elegans</i> |
| spellingShingle | Chen Zeng Shiling Feng The Antioxidant Capacity In Vitro and In Vivo of Polysaccharides From <i>Bergenia emeiensis</i> International Journal of Molecular Sciences <i>Bergenia emeiensis</i> polysaccharides antioxidant ability <i>C. elegans</i> |
| title | The Antioxidant Capacity In Vitro and In Vivo of Polysaccharides From <i>Bergenia emeiensis</i> |
| title_full | The Antioxidant Capacity In Vitro and In Vivo of Polysaccharides From <i>Bergenia emeiensis</i> |
| title_fullStr | The Antioxidant Capacity In Vitro and In Vivo of Polysaccharides From <i>Bergenia emeiensis</i> |
| title_full_unstemmed | The Antioxidant Capacity In Vitro and In Vivo of Polysaccharides From <i>Bergenia emeiensis</i> |
| title_short | The Antioxidant Capacity In Vitro and In Vivo of Polysaccharides From <i>Bergenia emeiensis</i> |
| title_sort | antioxidant capacity in vitro and in vivo of polysaccharides from i bergenia emeiensis i |
| topic | <i>Bergenia emeiensis</i> polysaccharides antioxidant ability <i>C. elegans</i> |
| url | https://www.mdpi.com/1422-0067/21/20/7456 |
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