Functional paclitaxel-manganese-doped mesoporous silica nanoparticles for orthotopic brain glioma targeted therapy
Brain glioma is a highly comorbid tumor with high mortality. In this study, plasma complex component-functionalized manganese-doped mesoporous silica nanoparticles (PMMSN) were prepared and loaded with paclitaxel (PTX) by adsorption for brain glioma treatment. The nanodrug delivery system crossed th...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-02-01
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| Series: | Materials & Design |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S026412752400087X |
| _version_ | 1797301621377466368 |
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| author | Ying Liu Shengxue Yu Xue Jiang Qian Wu Wenwen Shen Zhiru Zou Wei Wei Chao Wu Yu Gao |
| author_facet | Ying Liu Shengxue Yu Xue Jiang Qian Wu Wenwen Shen Zhiru Zou Wei Wei Chao Wu Yu Gao |
| author_sort | Ying Liu |
| collection | DOAJ |
| description | Brain glioma is a highly comorbid tumor with high mortality. In this study, plasma complex component-functionalized manganese-doped mesoporous silica nanoparticles (PMMSN) were prepared and loaded with paclitaxel (PTX) by adsorption for brain glioma treatment. The nanodrug delivery system crossed the blood–brain barrier (BBB) and exhibited redox responsiveness and pH-sensitive release characteristics. In vivo imaging revealed the ability of the preparation to cross the BBB, and oxidative stress studies revealed the redox properties of the carrier material. The drug release data indicated that the amount of drug released from the drug-loaded samples (PMMSN-PTX) in medium at pH 5.0 was 84.1 ± 1.62 % after 10 h, whereas it was 53.7 ± 1.53 % in pH 7.4 medium after 24 h. These findings demonstrated that the drug was pH sensitive and released slowly. In vitro cell-based assays, including cellular uptake, flow cytometry, western blotting, and immunofluorescence staining, showed that PMMSN-PTX facilitated drug delivery across the BBB and promoted tumor cell apoptosis. These in vivo antitumor effects further demonstrated that it significantly inhibited tumor cell proliferation. We believe that PMMSN-PTX is a promising bionic nanosystem agent that could be used as a new agent for treating glioma. |
| first_indexed | 2024-03-07T23:25:13Z |
| format | Article |
| id | doaj.art-dabfe1d127bf44df8ebeb1feef06639a |
| institution | Directory Open Access Journal |
| issn | 0264-1275 |
| language | English |
| last_indexed | 2024-03-07T23:25:13Z |
| publishDate | 2024-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials & Design |
| spelling | doaj.art-dabfe1d127bf44df8ebeb1feef06639a2024-02-21T05:24:15ZengElsevierMaterials & Design0264-12752024-02-01238112715Functional paclitaxel-manganese-doped mesoporous silica nanoparticles for orthotopic brain glioma targeted therapyYing Liu0Shengxue Yu1Xue Jiang2Qian Wu3Wenwen Shen4Zhiru Zou5Wei Wei6Chao Wu7Yu Gao8Pharmacy School, Jinzhou Medical University, Jinzhou, Liaoning 121001, ChinaLiaoning Key Laboratory of Diabetic Cognitive and Perceptive Dysfunction, Jinzhou Medical University, Jinzhou, Liaoning 121001, ChinaPharmacy School, Jinzhou Medical University, Jinzhou, Liaoning 121001, ChinaPharmacy School, Jinzhou Medical University, Jinzhou, Liaoning 121001, ChinaPharmacy School, Jinzhou Medical University, Jinzhou, Liaoning 121001, ChinaPharmacy School, Jinzhou Medical University, Jinzhou, Liaoning 121001, ChinaPharmacy School, Jinzhou Medical University, Jinzhou, Liaoning 121001, ChinaPharmacy School, Jinzhou Medical University, Jinzhou, Liaoning 121001, China; Corresponding authors.Department of Medical Oncology, the First Affiliated Hospital of Jinzhou Medical University, No. 2, the Fifth Section of Renmin Street, Guta District, Jinzhou, Liaoning Province 121001, China; Corresponding authors.Brain glioma is a highly comorbid tumor with high mortality. In this study, plasma complex component-functionalized manganese-doped mesoporous silica nanoparticles (PMMSN) were prepared and loaded with paclitaxel (PTX) by adsorption for brain glioma treatment. The nanodrug delivery system crossed the blood–brain barrier (BBB) and exhibited redox responsiveness and pH-sensitive release characteristics. In vivo imaging revealed the ability of the preparation to cross the BBB, and oxidative stress studies revealed the redox properties of the carrier material. The drug release data indicated that the amount of drug released from the drug-loaded samples (PMMSN-PTX) in medium at pH 5.0 was 84.1 ± 1.62 % after 10 h, whereas it was 53.7 ± 1.53 % in pH 7.4 medium after 24 h. These findings demonstrated that the drug was pH sensitive and released slowly. In vitro cell-based assays, including cellular uptake, flow cytometry, western blotting, and immunofluorescence staining, showed that PMMSN-PTX facilitated drug delivery across the BBB and promoted tumor cell apoptosis. These in vivo antitumor effects further demonstrated that it significantly inhibited tumor cell proliferation. We believe that PMMSN-PTX is a promising bionic nanosystem agent that could be used as a new agent for treating glioma.http://www.sciencedirect.com/science/article/pii/S026412752400087XManganese-doped mesoporous silica nanoparticlesOrthotopic brain gliomaPaclitaxelBlood–brain barrier |
| spellingShingle | Ying Liu Shengxue Yu Xue Jiang Qian Wu Wenwen Shen Zhiru Zou Wei Wei Chao Wu Yu Gao Functional paclitaxel-manganese-doped mesoporous silica nanoparticles for orthotopic brain glioma targeted therapy Materials & Design Manganese-doped mesoporous silica nanoparticles Orthotopic brain glioma Paclitaxel Blood–brain barrier |
| title | Functional paclitaxel-manganese-doped mesoporous silica nanoparticles for orthotopic brain glioma targeted therapy |
| title_full | Functional paclitaxel-manganese-doped mesoporous silica nanoparticles for orthotopic brain glioma targeted therapy |
| title_fullStr | Functional paclitaxel-manganese-doped mesoporous silica nanoparticles for orthotopic brain glioma targeted therapy |
| title_full_unstemmed | Functional paclitaxel-manganese-doped mesoporous silica nanoparticles for orthotopic brain glioma targeted therapy |
| title_short | Functional paclitaxel-manganese-doped mesoporous silica nanoparticles for orthotopic brain glioma targeted therapy |
| title_sort | functional paclitaxel manganese doped mesoporous silica nanoparticles for orthotopic brain glioma targeted therapy |
| topic | Manganese-doped mesoporous silica nanoparticles Orthotopic brain glioma Paclitaxel Blood–brain barrier |
| url | http://www.sciencedirect.com/science/article/pii/S026412752400087X |
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