Unconjugated Multi-Epitope Peptides Adjuvanted with ALFQ Induce Durable and Broadly Reactive Antibodies to Human and Avian Influenza Viruses
An unconjugated composite peptide vaccine targeting multiple conserved influenza epitopes from hemagglutinin, neuraminidase, and matrix protein and formulated with a safe and highly potent adjuvant, Army Liposome formulation (ALFQ), generated broad and durable immune responses in outbred mice. The a...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-09-01
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| Series: | Vaccines |
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| Online Access: | https://www.mdpi.com/2076-393X/11/9/1468 |
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| author | Nimisha Rikhi Clara J. Sei Mangala Rao Richard F. Schuman Kellie A. Kroscher Gary R. Matyas Kevin Muema Camille Lange Aba Assiaw-Dufu Elizabeth Hussin Ousman Jobe Carl R. Alving Gerald W. Fischer |
| author_facet | Nimisha Rikhi Clara J. Sei Mangala Rao Richard F. Schuman Kellie A. Kroscher Gary R. Matyas Kevin Muema Camille Lange Aba Assiaw-Dufu Elizabeth Hussin Ousman Jobe Carl R. Alving Gerald W. Fischer |
| author_sort | Nimisha Rikhi |
| collection | DOAJ |
| description | An unconjugated composite peptide vaccine targeting multiple conserved influenza epitopes from hemagglutinin, neuraminidase, and matrix protein and formulated with a safe and highly potent adjuvant, Army Liposome formulation (ALFQ), generated broad and durable immune responses in outbred mice. The antibodies recognized specific epitopes in influenza peptides and several human, avian, and swine influenza viruses. Comparable antibody responses to influenza viruses were observed with intramuscular and intradermal routes of vaccine administration. The peptide vaccine induced cross-reactive antibodies that recognized influenza virus subtypes A/H1N1, A/H3N2, A/H5N1, B/Victoria, and B/Yamagata. In addition, immune sera neutralized seasonal and pandemic influenza strains (Group 1 and Group 2). This composite multi-epitope peptide vaccine, formulated with ALFQ and administered via intramuscular and intradermal routes, provides a high-performance supra-seasonal vaccine that would be cost-effective and easily scalable, thus moving us closer to a viable strategy for a universal influenza vaccine and pandemic preparedness. |
| first_indexed | 2024-03-10T21:52:59Z |
| format | Article |
| id | doaj.art-dac232adba314c65a967ca9b2e8b7a16 |
| institution | Directory Open Access Journal |
| issn | 2076-393X |
| language | English |
| last_indexed | 2024-03-10T21:52:59Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Vaccines |
| spelling | doaj.art-dac232adba314c65a967ca9b2e8b7a162023-11-19T13:19:23ZengMDPI AGVaccines2076-393X2023-09-01119146810.3390/vaccines11091468Unconjugated Multi-Epitope Peptides Adjuvanted with ALFQ Induce Durable and Broadly Reactive Antibodies to Human and Avian Influenza VirusesNimisha Rikhi0Clara J. Sei1Mangala Rao2Richard F. Schuman3Kellie A. Kroscher4Gary R. Matyas5Kevin Muema6Camille Lange7Aba Assiaw-Dufu8Elizabeth Hussin9Ousman Jobe10Carl R. Alving11Gerald W. Fischer12Longhorn Vaccines and Diagnostics, Gaithersburg, MD 20878, USALonghorn Vaccines and Diagnostics, Gaithersburg, MD 20878, USAU.S. Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, MD 20910, USAAntibody and Immunoassay Consultants, Rockville, MD 20850, USALonghorn Vaccines and Diagnostics, Gaithersburg, MD 20878, USAU.S. Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, MD 20910, USALonghorn Vaccines and Diagnostics, Gaithersburg, MD 20878, USAU.S. Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, MD 20910, USALonghorn Vaccines and Diagnostics, Gaithersburg, MD 20878, USAU.S. Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, MD 20910, USAU.S. Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, MD 20910, USAU.S. Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, MD 20910, USALonghorn Vaccines and Diagnostics, Gaithersburg, MD 20878, USAAn unconjugated composite peptide vaccine targeting multiple conserved influenza epitopes from hemagglutinin, neuraminidase, and matrix protein and formulated with a safe and highly potent adjuvant, Army Liposome formulation (ALFQ), generated broad and durable immune responses in outbred mice. The antibodies recognized specific epitopes in influenza peptides and several human, avian, and swine influenza viruses. Comparable antibody responses to influenza viruses were observed with intramuscular and intradermal routes of vaccine administration. The peptide vaccine induced cross-reactive antibodies that recognized influenza virus subtypes A/H1N1, A/H3N2, A/H5N1, B/Victoria, and B/Yamagata. In addition, immune sera neutralized seasonal and pandemic influenza strains (Group 1 and Group 2). This composite multi-epitope peptide vaccine, formulated with ALFQ and administered via intramuscular and intradermal routes, provides a high-performance supra-seasonal vaccine that would be cost-effective and easily scalable, thus moving us closer to a viable strategy for a universal influenza vaccine and pandemic preparedness.https://www.mdpi.com/2076-393X/11/9/1468peptidesinfluenzaALFQliposomesQS21 (also known as QS-21)universal vaccine |
| spellingShingle | Nimisha Rikhi Clara J. Sei Mangala Rao Richard F. Schuman Kellie A. Kroscher Gary R. Matyas Kevin Muema Camille Lange Aba Assiaw-Dufu Elizabeth Hussin Ousman Jobe Carl R. Alving Gerald W. Fischer Unconjugated Multi-Epitope Peptides Adjuvanted with ALFQ Induce Durable and Broadly Reactive Antibodies to Human and Avian Influenza Viruses Vaccines peptides influenza ALFQ liposomes QS21 (also known as QS-21) universal vaccine |
| title | Unconjugated Multi-Epitope Peptides Adjuvanted with ALFQ Induce Durable and Broadly Reactive Antibodies to Human and Avian Influenza Viruses |
| title_full | Unconjugated Multi-Epitope Peptides Adjuvanted with ALFQ Induce Durable and Broadly Reactive Antibodies to Human and Avian Influenza Viruses |
| title_fullStr | Unconjugated Multi-Epitope Peptides Adjuvanted with ALFQ Induce Durable and Broadly Reactive Antibodies to Human and Avian Influenza Viruses |
| title_full_unstemmed | Unconjugated Multi-Epitope Peptides Adjuvanted with ALFQ Induce Durable and Broadly Reactive Antibodies to Human and Avian Influenza Viruses |
| title_short | Unconjugated Multi-Epitope Peptides Adjuvanted with ALFQ Induce Durable and Broadly Reactive Antibodies to Human and Avian Influenza Viruses |
| title_sort | unconjugated multi epitope peptides adjuvanted with alfq induce durable and broadly reactive antibodies to human and avian influenza viruses |
| topic | peptides influenza ALFQ liposomes QS21 (also known as QS-21) universal vaccine |
| url | https://www.mdpi.com/2076-393X/11/9/1468 |
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