A modular platform for automated cryo-FIB workflows

A modular platform for automated cryo-FIB workflows

Lamella micromachining by focused ion beam milling at cryogenic temperature (cryo-FIB) has matured into a preparation method widely used for cellular cryo-electron tomography. Due to the limited ablation rates of low Ga+ ion beam currents required to maintain the structural integrity of vitreous spe...

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Bibliographic Details
Main Authors: Sven Klumpe, Herman KH Fung, Sara K Goetz, Ievgeniia Zagoriy, Bernhard Hampoelz, Xiaojie Zhang, Philipp S Erdmann, Janina Baumbach, Christoph W Müller, Martin Beck, Jürgen M Plitzko, Julia Mahamid
Format: Article
Language:English
Published: eLife Sciences Publications Ltd 2021-12-01
Series:eLife
Subjects:
cryo-electron tomography
correlative light and electron microscopy
cryo-FIB-SEM
automation
in situ structural biology
electron microscopy
Online Access:https://elifesciences.org/articles/70506

Internet

https://elifesciences.org/articles/70506

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