Insoluble Fiber in Barley Leaf Attenuates Hyperuricemic Nephropathy by Modulating Gut Microbiota and Short-Chain Fatty Acids
Hyperuricemia (HUA), characterized by abnormal serum uric acid (UA) levels, is recognized as an important risk factor for hyperuricemic nephropathy (HN), which is strongly linked to gut microbiota. This study investigated the protective effects and regulatory mechanisms of insoluble fiber from barle...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-11-01
|
| Series: | Foods |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2304-8158/11/21/3482 |
| _version_ | 1797468328159084544 |
|---|---|
| author | Yongmei Li Lu Li Jinhong Tian Fengxin Zheng Hui Liao Zean Zhao Yanyu Chen Jianxin Pang Ting Wu |
| author_facet | Yongmei Li Lu Li Jinhong Tian Fengxin Zheng Hui Liao Zean Zhao Yanyu Chen Jianxin Pang Ting Wu |
| author_sort | Yongmei Li |
| collection | DOAJ |
| description | Hyperuricemia (HUA), characterized by abnormal serum uric acid (UA) levels, is recognized as an important risk factor for hyperuricemic nephropathy (HN), which is strongly linked to gut microbiota. This study investigated the protective effects and regulatory mechanisms of insoluble fiber from barley leaves (BL) against HN, induced by adenine (Ad) and potassium oxonate (PO). The results showed that BL dramatically reduced the levels of serum UA and creatinine (CR) and alleviated renal injury and fibrosis. Moreover, BL modulated oxidative stress and downregulated the expression of urate transporter 1 (URAT1) and glucose transporter 9 (GLUT9) in the kidneys of mice with HN. In addition, the 16S rRNA sequence data showed that BL also increased the relative abundance of short-chain fatty acids (SCFAs)-producing bacteria, including <i>Bacteroides</i>, <i>Alloprevotella</i>, and <i>Eisenbergiella</i>. Besides, BL treatment also increased SCFAs levels. Of interest, the application of SCFAs in hyperuricemic mice effectively reduced their serum UA. Furthermore, SCFAs dose-dependently inhibited URAT1 and GLUT9 in vitro and potently interacted with URAT1 and GLUT9 in the docking analysis. When taken together, our results indicate that BL and its metabolite SCFAs may be potential candidates for relieving HUA or HN. |
| first_indexed | 2024-03-09T19:04:54Z |
| format | Article |
| id | doaj.art-dacaf658df97459588f10340b31c2b27 |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-09T19:04:54Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-dacaf658df97459588f10340b31c2b272023-11-24T04:41:31ZengMDPI AGFoods2304-81582022-11-011121348210.3390/foods11213482Insoluble Fiber in Barley Leaf Attenuates Hyperuricemic Nephropathy by Modulating Gut Microbiota and Short-Chain Fatty AcidsYongmei Li0Lu Li1Jinhong Tian2Fengxin Zheng3Hui Liao4Zean Zhao5Yanyu Chen6Jianxin Pang7Ting Wu8Guangdong Provincial Key Laboratory of Drug Screening, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, ChinaGuangdong Provincial Key Laboratory of Drug Screening, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, ChinaGuangdong Provincial Key Laboratory of Drug Screening, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, ChinaGuangdong Provincial Key Laboratory of Drug Screening, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, ChinaGuangdong Provincial Key Laboratory of Drug Screening, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, ChinaGuangdong Provincial Key Laboratory of Drug Screening, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, ChinaGuangdong Provincial Key Laboratory of Drug Screening, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, ChinaGuangdong Provincial Key Laboratory of Drug Screening, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, ChinaGuangdong Provincial Key Laboratory of Drug Screening, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, ChinaHyperuricemia (HUA), characterized by abnormal serum uric acid (UA) levels, is recognized as an important risk factor for hyperuricemic nephropathy (HN), which is strongly linked to gut microbiota. This study investigated the protective effects and regulatory mechanisms of insoluble fiber from barley leaves (BL) against HN, induced by adenine (Ad) and potassium oxonate (PO). The results showed that BL dramatically reduced the levels of serum UA and creatinine (CR) and alleviated renal injury and fibrosis. Moreover, BL modulated oxidative stress and downregulated the expression of urate transporter 1 (URAT1) and glucose transporter 9 (GLUT9) in the kidneys of mice with HN. In addition, the 16S rRNA sequence data showed that BL also increased the relative abundance of short-chain fatty acids (SCFAs)-producing bacteria, including <i>Bacteroides</i>, <i>Alloprevotella</i>, and <i>Eisenbergiella</i>. Besides, BL treatment also increased SCFAs levels. Of interest, the application of SCFAs in hyperuricemic mice effectively reduced their serum UA. Furthermore, SCFAs dose-dependently inhibited URAT1 and GLUT9 in vitro and potently interacted with URAT1 and GLUT9 in the docking analysis. When taken together, our results indicate that BL and its metabolite SCFAs may be potential candidates for relieving HUA or HN.https://www.mdpi.com/2304-8158/11/21/3482barley leafhyperuricemic nephropathygut microbiotaSCFAsURAT1GLUT9 |
| spellingShingle | Yongmei Li Lu Li Jinhong Tian Fengxin Zheng Hui Liao Zean Zhao Yanyu Chen Jianxin Pang Ting Wu Insoluble Fiber in Barley Leaf Attenuates Hyperuricemic Nephropathy by Modulating Gut Microbiota and Short-Chain Fatty Acids Foods barley leaf hyperuricemic nephropathy gut microbiota SCFAs URAT1 GLUT9 |
| title | Insoluble Fiber in Barley Leaf Attenuates Hyperuricemic Nephropathy by Modulating Gut Microbiota and Short-Chain Fatty Acids |
| title_full | Insoluble Fiber in Barley Leaf Attenuates Hyperuricemic Nephropathy by Modulating Gut Microbiota and Short-Chain Fatty Acids |
| title_fullStr | Insoluble Fiber in Barley Leaf Attenuates Hyperuricemic Nephropathy by Modulating Gut Microbiota and Short-Chain Fatty Acids |
| title_full_unstemmed | Insoluble Fiber in Barley Leaf Attenuates Hyperuricemic Nephropathy by Modulating Gut Microbiota and Short-Chain Fatty Acids |
| title_short | Insoluble Fiber in Barley Leaf Attenuates Hyperuricemic Nephropathy by Modulating Gut Microbiota and Short-Chain Fatty Acids |
| title_sort | insoluble fiber in barley leaf attenuates hyperuricemic nephropathy by modulating gut microbiota and short chain fatty acids |
| topic | barley leaf hyperuricemic nephropathy gut microbiota SCFAs URAT1 GLUT9 |
| url | https://www.mdpi.com/2304-8158/11/21/3482 |
| work_keys_str_mv | AT yongmeili insolublefiberinbarleyleafattenuateshyperuricemicnephropathybymodulatinggutmicrobiotaandshortchainfattyacids AT luli insolublefiberinbarleyleafattenuateshyperuricemicnephropathybymodulatinggutmicrobiotaandshortchainfattyacids AT jinhongtian insolublefiberinbarleyleafattenuateshyperuricemicnephropathybymodulatinggutmicrobiotaandshortchainfattyacids AT fengxinzheng insolublefiberinbarleyleafattenuateshyperuricemicnephropathybymodulatinggutmicrobiotaandshortchainfattyacids AT huiliao insolublefiberinbarleyleafattenuateshyperuricemicnephropathybymodulatinggutmicrobiotaandshortchainfattyacids AT zeanzhao insolublefiberinbarleyleafattenuateshyperuricemicnephropathybymodulatinggutmicrobiotaandshortchainfattyacids AT yanyuchen insolublefiberinbarleyleafattenuateshyperuricemicnephropathybymodulatinggutmicrobiotaandshortchainfattyacids AT jianxinpang insolublefiberinbarleyleafattenuateshyperuricemicnephropathybymodulatinggutmicrobiotaandshortchainfattyacids AT tingwu insolublefiberinbarleyleafattenuateshyperuricemicnephropathybymodulatinggutmicrobiotaandshortchainfattyacids |