Validation of a high throughput screening assay to identify small molecules that target the eukaryotic replicative helicase

Validation of a high throughput screening assay to identify small molecules that target the eukaryotic replicative helicase

Mcm2-7 is the catalytic core of the eukaryotic replicative helicase, which together with CDC45 and the GINS complex unwind parental DNA to generate templates for DNA polymerase. Being a highly regulated and complex enzyme that operates via an incompletely understood multi-step mechanism, molecular p...

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Bibliographic Details
Main Authors: Jordan Sanders, Michael Castiglione, Tongying Shun, Laura L. Vollmer, Mark E. Schurdak, Andreas Vogt, Anthony Schwacha
Format: Article
Language:English
Published: Elsevier 2022-06-01
Series:SLAS Discovery
Subjects:
HTS assay development
DNA replication
Mcm2–7
Multivariate analysis
Yeast screening technology
Online Access:http://www.sciencedirect.com/science/article/pii/S2472555221000253

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http://www.sciencedirect.com/science/article/pii/S2472555221000253

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