Sex-specific effect of P2Y2 purinergic receptor on glucose metabolism during acute inflammation
The sex of an animal impacts glucose sensitivity, but little information is available regarding the mechanisms causing that difference, especially during acute inflammation. We examined sex-specific differences in the role of the P2Y2 receptor (P2Y2R) in glucose flux with and without LPS challenge....
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2023-08-01
|
| Series: | Frontiers in Endocrinology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fendo.2023.1248139/full |
| _version_ | 1797735970432352256 |
|---|---|
| author | Randi J. Ulbricht Christian A. Rivas Hailee Marino Erin Snyder Dana James Jamila Makhloufi Nathan Johnson Scott Zimmerman Jianjie Wang |
| author_facet | Randi J. Ulbricht Christian A. Rivas Hailee Marino Erin Snyder Dana James Jamila Makhloufi Nathan Johnson Scott Zimmerman Jianjie Wang |
| author_sort | Randi J. Ulbricht |
| collection | DOAJ |
| description | The sex of an animal impacts glucose sensitivity, but little information is available regarding the mechanisms causing that difference, especially during acute inflammation. We examined sex-specific differences in the role of the P2Y2 receptor (P2Y2R) in glucose flux with and without LPS challenge. Male and female wild-type and P2Y2R knockout mice (P2Y2R-/-) were injected with LPS or saline and glucose tolerance tests (GTT) were performed. P2Y2R, insulin receptor, and GLUT4 transporter gene expression was also evaluated. Female mice had reduced fasting plasma glucose and females had reduced glucose excursion times compared to male mice during GTT. P2Y2R-/- males had significantly decreased glucose flux throughout the GTT as compared to all female mice. Acute inflammation reduced fasting plasma glucose and the GTT area under the curve in both sexes. While both wild-type and P2Y2R-/- male animals displayed reduced fasting glucose in LPS treatment, female mice did not have significant difference in glucose tolerance, suggesting that the effects of P2Y2R are specific to male mice, even under inflammatory conditions. Overall, we conclude that the role for the purinergic receptor, P2Y2R, in regulating glucose metabolism is minimal in females but plays a large role in male mice, particularly in the acute inflammatory state. |
| first_indexed | 2024-03-12T13:06:49Z |
| format | Article |
| id | doaj.art-dad484205cfa45f4b464dc0b7d0ec90e |
| institution | Directory Open Access Journal |
| issn | 1664-2392 |
| language | English |
| last_indexed | 2024-03-12T13:06:49Z |
| publishDate | 2023-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Endocrinology |
| spelling | doaj.art-dad484205cfa45f4b464dc0b7d0ec90e2023-08-28T15:09:53ZengFrontiers Media S.A.Frontiers in Endocrinology1664-23922023-08-011410.3389/fendo.2023.12481391248139Sex-specific effect of P2Y2 purinergic receptor on glucose metabolism during acute inflammationRandi J. UlbrichtChristian A. RivasHailee MarinoErin SnyderDana JamesJamila MakhloufiNathan JohnsonScott ZimmermanJianjie WangThe sex of an animal impacts glucose sensitivity, but little information is available regarding the mechanisms causing that difference, especially during acute inflammation. We examined sex-specific differences in the role of the P2Y2 receptor (P2Y2R) in glucose flux with and without LPS challenge. Male and female wild-type and P2Y2R knockout mice (P2Y2R-/-) were injected with LPS or saline and glucose tolerance tests (GTT) were performed. P2Y2R, insulin receptor, and GLUT4 transporter gene expression was also evaluated. Female mice had reduced fasting plasma glucose and females had reduced glucose excursion times compared to male mice during GTT. P2Y2R-/- males had significantly decreased glucose flux throughout the GTT as compared to all female mice. Acute inflammation reduced fasting plasma glucose and the GTT area under the curve in both sexes. While both wild-type and P2Y2R-/- male animals displayed reduced fasting glucose in LPS treatment, female mice did not have significant difference in glucose tolerance, suggesting that the effects of P2Y2R are specific to male mice, even under inflammatory conditions. Overall, we conclude that the role for the purinergic receptor, P2Y2R, in regulating glucose metabolism is minimal in females but plays a large role in male mice, particularly in the acute inflammatory state.https://www.frontiersin.org/articles/10.3389/fendo.2023.1248139/fullpurinergic signalingglucose homeostasisP2Y receptorinflammationsex-dependence |
| spellingShingle | Randi J. Ulbricht Christian A. Rivas Hailee Marino Erin Snyder Dana James Jamila Makhloufi Nathan Johnson Scott Zimmerman Jianjie Wang Sex-specific effect of P2Y2 purinergic receptor on glucose metabolism during acute inflammation Frontiers in Endocrinology purinergic signaling glucose homeostasis P2Y receptor inflammation sex-dependence |
| title | Sex-specific effect of P2Y2 purinergic receptor on glucose metabolism during acute inflammation |
| title_full | Sex-specific effect of P2Y2 purinergic receptor on glucose metabolism during acute inflammation |
| title_fullStr | Sex-specific effect of P2Y2 purinergic receptor on glucose metabolism during acute inflammation |
| title_full_unstemmed | Sex-specific effect of P2Y2 purinergic receptor on glucose metabolism during acute inflammation |
| title_short | Sex-specific effect of P2Y2 purinergic receptor on glucose metabolism during acute inflammation |
| title_sort | sex specific effect of p2y2 purinergic receptor on glucose metabolism during acute inflammation |
| topic | purinergic signaling glucose homeostasis P2Y receptor inflammation sex-dependence |
| url | https://www.frontiersin.org/articles/10.3389/fendo.2023.1248139/full |
| work_keys_str_mv | AT randijulbricht sexspecificeffectofp2y2purinergicreceptoronglucosemetabolismduringacuteinflammation AT christianarivas sexspecificeffectofp2y2purinergicreceptoronglucosemetabolismduringacuteinflammation AT haileemarino sexspecificeffectofp2y2purinergicreceptoronglucosemetabolismduringacuteinflammation AT erinsnyder sexspecificeffectofp2y2purinergicreceptoronglucosemetabolismduringacuteinflammation AT danajames sexspecificeffectofp2y2purinergicreceptoronglucosemetabolismduringacuteinflammation AT jamilamakhloufi sexspecificeffectofp2y2purinergicreceptoronglucosemetabolismduringacuteinflammation AT nathanjohnson sexspecificeffectofp2y2purinergicreceptoronglucosemetabolismduringacuteinflammation AT scottzimmerman sexspecificeffectofp2y2purinergicreceptoronglucosemetabolismduringacuteinflammation AT jianjiewang sexspecificeffectofp2y2purinergicreceptoronglucosemetabolismduringacuteinflammation |