Valorization of Grape Pomace and Berries as a New and Sustainable Dietary Supplement: Development, Characterization, and Antioxidant Activity Testing
Grape pomace and berries represent natural sources of phytochemicals that can increase the quality of life of consumers by contributing to the prevention of chronic diseases; thus, the development of a dietary supplement was necessary. The raw material (r.m.) used for the development of the dietary...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-07-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/14/15/3065 |
| _version_ | 1797432800613236736 |
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| author | Adina Frum Carmen Maximiliana Dobrea Luca Liviu Rus Lidia-Ioana Virchea Claudiu Morgovan Adriana Aurelia Chis Anca Maria Arseniu Anca Butuca Felicia Gabriela Gligor Laura Gratiela Vicas Ovidiu Tita Cecilia Georgescu |
| author_facet | Adina Frum Carmen Maximiliana Dobrea Luca Liviu Rus Lidia-Ioana Virchea Claudiu Morgovan Adriana Aurelia Chis Anca Maria Arseniu Anca Butuca Felicia Gabriela Gligor Laura Gratiela Vicas Ovidiu Tita Cecilia Georgescu |
| author_sort | Adina Frum |
| collection | DOAJ |
| description | Grape pomace and berries represent natural sources of phytochemicals that can increase the quality of life of consumers by contributing to the prevention of chronic diseases; thus, the development of a dietary supplement was necessary. The raw material (r.m.) used for the development of the dietary supplement consisted of dried and powdered bilberries (<i>Vaccinium myrtillus</i> L.), red currants (<i>Ribes rubrum</i> L.), and red fermented pomaces (<i>Vitis vinifera</i> L.) from Feteasca Neagra and Cabernet Sauvignon cultivars. The particle size distribution, powder flow, total phenolic content (TPC), HPLC-DAD phenolic profile assessment, and radical scavenging assay (RSA) were employed for the analysis of the raw material. After encapsulation, the average mass and uniformity of mass, the disintegration, and the uniformity of content for the obtained capsules were performed to obtain a high-quality dietary supplement. All the assays performed complied to the compendial requirements and the TPC was determined at 9.07 ± 0.25 mg gallic acid equivalents/g r.m. and RSA at 48.32 ± 0.74%. The highest quantities of phenolic compounds determined were 333.7 ± 0.50 µg/g r.m. for chlorogenic acid, followed by rutin, ferulic acid, and (+)-catechin with 198.9 ± 1.60 µg/g r.m., 179.8 ± 0.90 µg/g r.m. and 118.7 ± 0.75 µg/g r.m., respectively. The results of this study can be used for the manufacturing and assessing of pilot scale-up capsule batches and thinking of quality assurance, we recommend that the industrial batch extracts should be standardized in polyphenols, and the manufacturing process should be validated. |
| first_indexed | 2024-03-09T10:06:54Z |
| format | Article |
| id | doaj.art-dad4e4d8e24d4a6194ca94a75dda44a3 |
| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-09T10:06:54Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-dad4e4d8e24d4a6194ca94a75dda44a32023-12-01T23:05:12ZengMDPI AGNutrients2072-66432022-07-011415306510.3390/nu14153065Valorization of Grape Pomace and Berries as a New and Sustainable Dietary Supplement: Development, Characterization, and Antioxidant Activity TestingAdina Frum0Carmen Maximiliana Dobrea1Luca Liviu Rus2Lidia-Ioana Virchea3Claudiu Morgovan4Adriana Aurelia Chis5Anca Maria Arseniu6Anca Butuca7Felicia Gabriela Gligor8Laura Gratiela Vicas9Ovidiu Tita10Cecilia Georgescu11Faculty of Medicine, “Lucian Blaga” University of Sibiu, 550169 Sibiu, RomaniaFaculty of Medicine, “Lucian Blaga” University of Sibiu, 550169 Sibiu, RomaniaFaculty of Medicine, “Lucian Blaga” University of Sibiu, 550169 Sibiu, RomaniaFaculty of Medicine, “Lucian Blaga” University of Sibiu, 550169 Sibiu, RomaniaFaculty of Medicine, “Lucian Blaga” University of Sibiu, 550169 Sibiu, RomaniaFaculty of Medicine, “Lucian Blaga” University of Sibiu, 550169 Sibiu, RomaniaFaculty of Medicine, “Lucian Blaga” University of Sibiu, 550169 Sibiu, RomaniaFaculty of Medicine, “Lucian Blaga” University of Sibiu, 550169 Sibiu, RomaniaFaculty of Medicine, “Lucian Blaga” University of Sibiu, 550169 Sibiu, RomaniaFaculty of Medicine and Pharmacy, University of Oradea, 410028 Oradea, RomaniaFaculty of Agriculture Science, Food Industry and Environmental Protection, “Lucian Blaga” University of Sibiu, 550012 Sibiu, RomaniaFaculty of Agriculture Science, Food Industry and Environmental Protection, “Lucian Blaga” University of Sibiu, 550012 Sibiu, RomaniaGrape pomace and berries represent natural sources of phytochemicals that can increase the quality of life of consumers by contributing to the prevention of chronic diseases; thus, the development of a dietary supplement was necessary. The raw material (r.m.) used for the development of the dietary supplement consisted of dried and powdered bilberries (<i>Vaccinium myrtillus</i> L.), red currants (<i>Ribes rubrum</i> L.), and red fermented pomaces (<i>Vitis vinifera</i> L.) from Feteasca Neagra and Cabernet Sauvignon cultivars. The particle size distribution, powder flow, total phenolic content (TPC), HPLC-DAD phenolic profile assessment, and radical scavenging assay (RSA) were employed for the analysis of the raw material. After encapsulation, the average mass and uniformity of mass, the disintegration, and the uniformity of content for the obtained capsules were performed to obtain a high-quality dietary supplement. All the assays performed complied to the compendial requirements and the TPC was determined at 9.07 ± 0.25 mg gallic acid equivalents/g r.m. and RSA at 48.32 ± 0.74%. The highest quantities of phenolic compounds determined were 333.7 ± 0.50 µg/g r.m. for chlorogenic acid, followed by rutin, ferulic acid, and (+)-catechin with 198.9 ± 1.60 µg/g r.m., 179.8 ± 0.90 µg/g r.m. and 118.7 ± 0.75 µg/g r.m., respectively. The results of this study can be used for the manufacturing and assessing of pilot scale-up capsule batches and thinking of quality assurance, we recommend that the industrial batch extracts should be standardized in polyphenols, and the manufacturing process should be validated.https://www.mdpi.com/2072-6643/14/15/3065antioxidantsbilberryby-productsfood supplementHPLCphenolic compounds |
| spellingShingle | Adina Frum Carmen Maximiliana Dobrea Luca Liviu Rus Lidia-Ioana Virchea Claudiu Morgovan Adriana Aurelia Chis Anca Maria Arseniu Anca Butuca Felicia Gabriela Gligor Laura Gratiela Vicas Ovidiu Tita Cecilia Georgescu Valorization of Grape Pomace and Berries as a New and Sustainable Dietary Supplement: Development, Characterization, and Antioxidant Activity Testing Nutrients antioxidants bilberry by-products food supplement HPLC phenolic compounds |
| title | Valorization of Grape Pomace and Berries as a New and Sustainable Dietary Supplement: Development, Characterization, and Antioxidant Activity Testing |
| title_full | Valorization of Grape Pomace and Berries as a New and Sustainable Dietary Supplement: Development, Characterization, and Antioxidant Activity Testing |
| title_fullStr | Valorization of Grape Pomace and Berries as a New and Sustainable Dietary Supplement: Development, Characterization, and Antioxidant Activity Testing |
| title_full_unstemmed | Valorization of Grape Pomace and Berries as a New and Sustainable Dietary Supplement: Development, Characterization, and Antioxidant Activity Testing |
| title_short | Valorization of Grape Pomace and Berries as a New and Sustainable Dietary Supplement: Development, Characterization, and Antioxidant Activity Testing |
| title_sort | valorization of grape pomace and berries as a new and sustainable dietary supplement development characterization and antioxidant activity testing |
| topic | antioxidants bilberry by-products food supplement HPLC phenolic compounds |
| url | https://www.mdpi.com/2072-6643/14/15/3065 |
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