Extreme Diversity of IgGs Against Histones, DNA, and Myelin Basic Protein in the Cerebrospinal Fluid and Blood of Patients with Multiple Sclerosis
It was recently shown that IgGs from sera of multiple sclerosis (MS) patients are active in the hydrolysis of DNA and myelin basic protein (MBP). We first analyzed the relative concentration of antibodies against five histones (H1, H2a, H2b, H3, and H4) in the cerebrospinal fluid (CSF) and serum of...
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MDPI AG
2020-04-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/10/4/630 |
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| author | Irina A. Kostrikina Valentina N. Buneva Enrico Granieri Georgy A. Nevinsky |
| author_facet | Irina A. Kostrikina Valentina N. Buneva Enrico Granieri Georgy A. Nevinsky |
| author_sort | Irina A. Kostrikina |
| collection | DOAJ |
| description | It was recently shown that IgGs from sera of multiple sclerosis (MS) patients are active in the hydrolysis of DNA and myelin basic protein (MBP). We first analyzed the relative concentration of antibodies against five histones (H1, H2a, H2b, H3, and H4) in the cerebrospinal fluid (CSF) and serum of patients with MS. The relative concentrations of blood and CSF IgGs against histones and their activity in the hydrolysis of five histones varied greatly from patient to patient. However, all 28 IgG preparations were hydrolyzed from one to five histones. Relative activities and correlation coefficients among the activities of IgGs from serum and CSF in the hydrolysis of five histones (H1, H2a, H2b, H3, and H4), DNA, and MBP were calculated. It was shown that auto-IgGs from CSF and sera of MS patients are extremely heterogeneous in their affinity to histones, MBP, and DNA. The heterogeneity of IgG-abzymes hydrolyzing DNA, MBP, and histones from CSF and sera was also demonstrated using their isoelectrofocusing. The isofocusing profiles DNase, MBP-, and histone-hydrolyzing activities of IgGs may be very different for various individuals, but the total IgG subfractions with all their activities are distributed from pH 3 to 10. |
| first_indexed | 2024-03-10T20:22:41Z |
| format | Article |
| id | doaj.art-daf66d880c0645f4bc6fc0ebae026b8c |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-10T20:22:41Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-daf66d880c0645f4bc6fc0ebae026b8c2023-11-19T22:04:33ZengMDPI AGBiomolecules2218-273X2020-04-0110463010.3390/biom10040630Extreme Diversity of IgGs Against Histones, DNA, and Myelin Basic Protein in the Cerebrospinal Fluid and Blood of Patients with Multiple SclerosisIrina A. Kostrikina0Valentina N. Buneva1Enrico Granieri2Georgy A. Nevinsky3Institute of Chemical Biology and Fundamental Medicine, Russian Academy of Sciences, Siberian Division, 630090 Novosibirsk, RussiaInstitute of Chemical Biology and Fundamental Medicine, Russian Academy of Sciences, Siberian Division, 630090 Novosibirsk, RussiaMultiple Sclerosis Center, Department of Neurology, Ferrara University, 44121 Ferrara, ItalyInstitute of Chemical Biology and Fundamental Medicine, Russian Academy of Sciences, Siberian Division, 630090 Novosibirsk, RussiaIt was recently shown that IgGs from sera of multiple sclerosis (MS) patients are active in the hydrolysis of DNA and myelin basic protein (MBP). We first analyzed the relative concentration of antibodies against five histones (H1, H2a, H2b, H3, and H4) in the cerebrospinal fluid (CSF) and serum of patients with MS. The relative concentrations of blood and CSF IgGs against histones and their activity in the hydrolysis of five histones varied greatly from patient to patient. However, all 28 IgG preparations were hydrolyzed from one to five histones. Relative activities and correlation coefficients among the activities of IgGs from serum and CSF in the hydrolysis of five histones (H1, H2a, H2b, H3, and H4), DNA, and MBP were calculated. It was shown that auto-IgGs from CSF and sera of MS patients are extremely heterogeneous in their affinity to histones, MBP, and DNA. The heterogeneity of IgG-abzymes hydrolyzing DNA, MBP, and histones from CSF and sera was also demonstrated using their isoelectrofocusing. The isofocusing profiles DNase, MBP-, and histone-hydrolyzing activities of IgGs may be very different for various individuals, but the total IgG subfractions with all their activities are distributed from pH 3 to 10.https://www.mdpi.com/2218-273X/10/4/630multiple sclerosishuman cerebrospinal fluidcatalytic IgGsDNase activityprotease activityextreme diversity of IgGs against auto-antigens |
| spellingShingle | Irina A. Kostrikina Valentina N. Buneva Enrico Granieri Georgy A. Nevinsky Extreme Diversity of IgGs Against Histones, DNA, and Myelin Basic Protein in the Cerebrospinal Fluid and Blood of Patients with Multiple Sclerosis Biomolecules multiple sclerosis human cerebrospinal fluid catalytic IgGs DNase activity protease activity extreme diversity of IgGs against auto-antigens |
| title | Extreme Diversity of IgGs Against Histones, DNA, and Myelin Basic Protein in the Cerebrospinal Fluid and Blood of Patients with Multiple Sclerosis |
| title_full | Extreme Diversity of IgGs Against Histones, DNA, and Myelin Basic Protein in the Cerebrospinal Fluid and Blood of Patients with Multiple Sclerosis |
| title_fullStr | Extreme Diversity of IgGs Against Histones, DNA, and Myelin Basic Protein in the Cerebrospinal Fluid and Blood of Patients with Multiple Sclerosis |
| title_full_unstemmed | Extreme Diversity of IgGs Against Histones, DNA, and Myelin Basic Protein in the Cerebrospinal Fluid and Blood of Patients with Multiple Sclerosis |
| title_short | Extreme Diversity of IgGs Against Histones, DNA, and Myelin Basic Protein in the Cerebrospinal Fluid and Blood of Patients with Multiple Sclerosis |
| title_sort | extreme diversity of iggs against histones dna and myelin basic protein in the cerebrospinal fluid and blood of patients with multiple sclerosis |
| topic | multiple sclerosis human cerebrospinal fluid catalytic IgGs DNase activity protease activity extreme diversity of IgGs against auto-antigens |
| url | https://www.mdpi.com/2218-273X/10/4/630 |
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