Spectrum-Effect Relationships Between the Bioactive Ingredient of Syringa oblata Lindl. Leaves and Its Role in Inhibiting the Biofilm Formation of Streptococcus suis
Syringa oblata Lindl. (S. oblata) has been used in herbal medicines for treating bacterial diseases. It is also thought to inhibit Streptococcus suis (S. suis) biofilm formation. However, due to the inherent nature of the complexity in its chemical properties, it is difficult to understand the possi...
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Frontiers Media S.A.
2018-06-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fphar.2018.00570/full |
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| author | Yan-Yan Liu Yan-Yan Liu Xing-Ru Chen Xing-Ru Chen Ling-Fei Gao Ling-Fei Gao Mo Chen Mo Chen Wen-Qiang Cui Wen-Qiang Cui Wen-Ya Ding Wen-Ya Ding Xue-Ying Chen Xue-Ying Chen Bello-Onaghise God’spower Bello-Onaghise God’spower Yan-Hua Li Yan-Hua Li |
| author_facet | Yan-Yan Liu Yan-Yan Liu Xing-Ru Chen Xing-Ru Chen Ling-Fei Gao Ling-Fei Gao Mo Chen Mo Chen Wen-Qiang Cui Wen-Qiang Cui Wen-Ya Ding Wen-Ya Ding Xue-Ying Chen Xue-Ying Chen Bello-Onaghise God’spower Bello-Onaghise God’spower Yan-Hua Li Yan-Hua Li |
| author_sort | Yan-Yan Liu |
| collection | DOAJ |
| description | Syringa oblata Lindl. (S. oblata) has been used in herbal medicines for treating bacterial diseases. It is also thought to inhibit Streptococcus suis (S. suis) biofilm formation. However, due to the inherent nature of the complexity in its chemical properties, it is difficult to understand the possible bioactive ingredients of S. oblata. The spectrum-effect relationships method was applied to screen the main active ingredients in S. oblata obtained from Heilongjiang Province based on gray relational analysis. The results revealed that Sub-MICs obtained from 10 batches of S. oblata could inhibit biofilm formation by S. suis. Gray relational analysis revealed variations in the contents of 15 main peaks and rutin was discovered to be the main active ingredient. Then, the function of rutin was further verified by inhibiting S. suis biofilm formation using crystal violet staining. Computational studies revealed that rutin may target the chloramphenicol acetyltransferase protein in the biofilm formation of S. suis. In conclusion, this study revealed that the spectrum-effect relationships and computational studies are useful tools to associate the active ingredient with the potential anti-biofilm effects of S. oblata. Here, our findings would provide foundation for the further understanding of the mechanism of S. oblata intervention in biofilm formation. |
| first_indexed | 2024-12-16T12:56:14Z |
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| id | doaj.art-dafb40add4654168bfb99fc35f483fed |
| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-12-16T12:56:14Z |
| publishDate | 2018-06-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Pharmacology |
| spelling | doaj.art-dafb40add4654168bfb99fc35f483fed2022-12-21T22:31:01ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122018-06-01910.3389/fphar.2018.00570367345Spectrum-Effect Relationships Between the Bioactive Ingredient of Syringa oblata Lindl. Leaves and Its Role in Inhibiting the Biofilm Formation of Streptococcus suisYan-Yan Liu0Yan-Yan Liu1Xing-Ru Chen2Xing-Ru Chen3Ling-Fei Gao4Ling-Fei Gao5Mo Chen6Mo Chen7Wen-Qiang Cui8Wen-Qiang Cui9Wen-Ya Ding10Wen-Ya Ding11Xue-Ying Chen12Xue-Ying Chen13Bello-Onaghise God’spower14Bello-Onaghise God’spower15Yan-Hua Li16Yan-Hua Li17College of Veterinary Medicine, Northeast Agricultural University, Harbin, ChinaHeilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development, Harbin, ChinaCollege of Veterinary Medicine, Northeast Agricultural University, Harbin, ChinaHeilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development, Harbin, ChinaCollege of Veterinary Medicine, Northeast Agricultural University, Harbin, ChinaHeilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development, Harbin, ChinaCollege of Veterinary Medicine, Northeast Agricultural University, Harbin, ChinaHeilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development, Harbin, ChinaCollege of Veterinary Medicine, Northeast Agricultural University, Harbin, ChinaHeilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development, Harbin, ChinaCollege of Veterinary Medicine, Northeast Agricultural University, Harbin, ChinaHeilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development, Harbin, ChinaCollege of Veterinary Medicine, Northeast Agricultural University, Harbin, ChinaHeilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development, Harbin, ChinaCollege of Veterinary Medicine, Northeast Agricultural University, Harbin, ChinaHeilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development, Harbin, ChinaCollege of Veterinary Medicine, Northeast Agricultural University, Harbin, ChinaHeilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development, Harbin, ChinaSyringa oblata Lindl. (S. oblata) has been used in herbal medicines for treating bacterial diseases. It is also thought to inhibit Streptococcus suis (S. suis) biofilm formation. However, due to the inherent nature of the complexity in its chemical properties, it is difficult to understand the possible bioactive ingredients of S. oblata. The spectrum-effect relationships method was applied to screen the main active ingredients in S. oblata obtained from Heilongjiang Province based on gray relational analysis. The results revealed that Sub-MICs obtained from 10 batches of S. oblata could inhibit biofilm formation by S. suis. Gray relational analysis revealed variations in the contents of 15 main peaks and rutin was discovered to be the main active ingredient. Then, the function of rutin was further verified by inhibiting S. suis biofilm formation using crystal violet staining. Computational studies revealed that rutin may target the chloramphenicol acetyltransferase protein in the biofilm formation of S. suis. In conclusion, this study revealed that the spectrum-effect relationships and computational studies are useful tools to associate the active ingredient with the potential anti-biofilm effects of S. oblata. Here, our findings would provide foundation for the further understanding of the mechanism of S. oblata intervention in biofilm formation.https://www.frontiersin.org/article/10.3389/fphar.2018.00570/fullSyringa oblata Lindl.spectrum-effect relationshipsactive ingredientsbiofilm formationcomputational studies |
| spellingShingle | Yan-Yan Liu Yan-Yan Liu Xing-Ru Chen Xing-Ru Chen Ling-Fei Gao Ling-Fei Gao Mo Chen Mo Chen Wen-Qiang Cui Wen-Qiang Cui Wen-Ya Ding Wen-Ya Ding Xue-Ying Chen Xue-Ying Chen Bello-Onaghise God’spower Bello-Onaghise God’spower Yan-Hua Li Yan-Hua Li Spectrum-Effect Relationships Between the Bioactive Ingredient of Syringa oblata Lindl. Leaves and Its Role in Inhibiting the Biofilm Formation of Streptococcus suis Frontiers in Pharmacology Syringa oblata Lindl. spectrum-effect relationships active ingredients biofilm formation computational studies |
| title | Spectrum-Effect Relationships Between the Bioactive Ingredient of Syringa oblata Lindl. Leaves and Its Role in Inhibiting the Biofilm Formation of Streptococcus suis |
| title_full | Spectrum-Effect Relationships Between the Bioactive Ingredient of Syringa oblata Lindl. Leaves and Its Role in Inhibiting the Biofilm Formation of Streptococcus suis |
| title_fullStr | Spectrum-Effect Relationships Between the Bioactive Ingredient of Syringa oblata Lindl. Leaves and Its Role in Inhibiting the Biofilm Formation of Streptococcus suis |
| title_full_unstemmed | Spectrum-Effect Relationships Between the Bioactive Ingredient of Syringa oblata Lindl. Leaves and Its Role in Inhibiting the Biofilm Formation of Streptococcus suis |
| title_short | Spectrum-Effect Relationships Between the Bioactive Ingredient of Syringa oblata Lindl. Leaves and Its Role in Inhibiting the Biofilm Formation of Streptococcus suis |
| title_sort | spectrum effect relationships between the bioactive ingredient of syringa oblata lindl leaves and its role in inhibiting the biofilm formation of streptococcus suis |
| topic | Syringa oblata Lindl. spectrum-effect relationships active ingredients biofilm formation computational studies |
| url | https://www.frontiersin.org/article/10.3389/fphar.2018.00570/full |
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