GST-Perfringolysin O production for the localization and quantification of membrane cholesterol in human and mouse brain and liver

GST-Perfringolysin O production for the localization and quantification of membrane cholesterol in human and mouse brain and liver

Summary: Abnormal cholesterol metabolism is linked to many neurodegenerative disorders. Here, we present a protocol for the production of a recombinant protein consisting of a Glutathione-S-Transferase tag fused with the Perfringolysin O (PFO). The GST-PFO tag enables analysis of the localization of...

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Bibliographic Details
Main Authors: Leire Goicoechea, Fabian Arenas, Fernanda Castro, Susana Nuñez, Sandra Torres, Carmen Garcia-Ruiz, José C. Fernandez-Checa
Format: Article
Language:English
Published: Elsevier 2022-03-01
Series:STAR Protocols
Subjects:
Cell Biology
Cell Membrane
Health Sciences
Metabolism
Microscopy
Model Organisms
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721007747

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http://www.sciencedirect.com/science/article/pii/S2666166721007747

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