Boosting genome editing efficiency in human cells and plants with novel LbCas12a variants

Boosting genome editing efficiency in human cells and plants with novel LbCas12a variants

Abstract Background Cas12a (formerly known as Cpf1), the class II type V CRISPR nuclease, has been widely used for genome editing in mammalian cells and plants due to its distinct characteristics from Cas9. Despite being one of the most robust Cas12a nucleases, LbCas12a in general is less efficient...

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Bibliographic Details
Main Authors: Liyang Zhang, Gen Li, Yingxiao Zhang, Yanhao Cheng, Nathaniel Roberts, Steve E. Glenn, Diane DeZwaan-McCabe, H. Tomas Rube, Jeff Manthey, Gary Coleman, Christopher A. Vakulskas, Yiping Qi
Format: Article
Language:English
Published: BMC 2023-04-01
Series:Genome Biology
Subjects:
Protein evolution
LbCas12a-RVQ
LbCas12a-RV
LbCas12a-RRV
Genome editing
Human cells
Online Access:https://doi.org/10.1186/s13059-023-02929-6

Internet

https://doi.org/10.1186/s13059-023-02929-6

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