Purification of Escherichia coli-expressed HIS-tagged Maedi-Visna p25 core antigen by Ni2+-chelate affinity chromatography

In this study, recombinant histidine tagged p25 capsid protein of Maedi-Visna virus was developed. Part of the viral genome coding p25 protein was positioned downstream and in frame with a metal binding domain in pRSET-B vector. Recombinant protein was expressed in E. coli cells and soluble fraction...

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Bibliografische gegevens
Hoofdauteur: D. Molinková
Formaat: Artikel
Taal:English
Gepubliceerd in: Czech Academy of Agricultural Sciences 2001-02-01
Reeks:Veterinární Medicína
Onderwerpen:
Online toegang:https://vetmed.agriculturejournals.cz/artkey/vet-200102-0005_purification-of-escherichia-coli-expressed-his-tagged-maedi-visna-p25-core-antigen-by-ni2-chelate-affinity-chr.php