A Three-Dimensional Model of the Yeast Transmembrane Sensor Wsc1 Obtained by SMA-Based Detergent-Free Purification and Transmission Electron Microscopy

A Three-Dimensional Model of the Yeast Transmembrane Sensor Wsc1 Obtained by SMA-Based Detergent-Free Purification and Transmission Electron Microscopy

The cell wall sensor Wsc1 belongs to a small family of transmembrane proteins, which are crucial to sustain cell integrity in yeast and other fungi. Wsc1 acts as a mechanosensor of the cell wall integrity (CWI) signal transduction pathway which responds to external stresses. Here we report on the pu...

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Bibliographic Details
Main Authors: Natalia Voskoboynikova, Maria Karlova, Rainer Kurre, Armen Y. Mulkidjanian, Konstantin V. Shaitan, Olga S. Sokolova, Heinz-Jürgen Steinhoff, Jürgen J. Heinisch
Format: Article
Language:English
Published: MDPI AG 2021-02-01
Series:Journal of Fungi
Subjects:
Wsc1
membrane sensor
SMALP
detergent-free extraction
fluorescence correlation spectroscopy
transmission electron microscopy
Online Access:https://www.mdpi.com/2309-608X/7/2/118

Internet

https://www.mdpi.com/2309-608X/7/2/118

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