Ov-RPA–CRISPR/Cas12a assay for the detection of Opisthorchis viverrini infection in field-collected human feces

Ov-RPA–CRISPR/Cas12a assay for the detection of Opisthorchis viverrini infection in field-collected human feces

Abstract Background Opisthorchis viverrini infection is traditionally diagnosed using the Kato–Katz method and formalin ethyl–acetate concentration technique. However, the limited sensitivity and specificity of these techniques have prompted the exploration of various molecular approaches, such as c...

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Bibliographic Details
Main Authors: Orawan Phuphisut, Akkarin Poodeepiyasawat, Tippayarat Yoonuan, Dorn Watthanakulpanich, Charin Thawornkuno, Onrapak Reamtong, Megumi Sato, Poom Adisakwattana
Format: Article
Language:English
Published: BMC 2024-02-01
Series:Parasites & Vectors
Subjects:
Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 12a (CRISPR/Cas12a)
Diagnostic method
Fecal sample
Opisthorchiasis
Opisthorchis viverrini
Recombinase polymerase amplification
Online Access:https://doi.org/10.1186/s13071-024-06134-7

Internet

https://doi.org/10.1186/s13071-024-06134-7

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