Composition analysis and structural identification of anthocyanins in fruit of waxberry
Anthocyanin pigments in the fruit of waxberry (Myrica rubra Sieb. et Zucc.), were extracted with 0.1% HCl in ethanol, and the crude anthocyanin extract was purified by C18 Sep-Pak cartridge open-column chromatography. High-performance liquid chromatography (HPLC) with photodiode array detection (PAD...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2011-04-01
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| Series: | Czech Journal of Food Sciences |
| Subjects: | |
| Online Access: | https://cjfs.agriculturejournals.cz/artkey/cjf-201102-0010_composition-analysis-and-structural-identification-of-anthocyanins-in-fruit-of-waxberry.php |
| _version_ | 1828009192358674432 |
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| author | Chuan-Guang Qin Yang Li Weining Niu Yan Ding Xiaoya Shang Chunlan Xu |
| author_facet | Chuan-Guang Qin Yang Li Weining Niu Yan Ding Xiaoya Shang Chunlan Xu |
| author_sort | Chuan-Guang Qin |
| collection | DOAJ |
| description | Anthocyanin pigments in the fruit of waxberry (Myrica rubra Sieb. et Zucc.), were extracted with 0.1% HCl in ethanol, and the crude anthocyanin extract was purified by C18 Sep-Pak cartridge open-column chromatography. High-performance liquid chromatography (HPLC) with photodiode array detection (PAD) and matrix-assisted laser desorption/ionisation-time of flight-mass spectrometry (MALDI-TOF-MS) was applied for the separation and identification of anthocyanins in the fruit of waxberry and their aglycones resulting from acid hydrolysis. Three anthocyanins were found in the fruit of waxberry and identified as Cyanidin 3-O-β-galacopyranoside (14.8%), Cyanidin 3-O-β-gluco-pyranoside (60.5%), and petunidin 3-O-β-glucopyranoside (24.7%), respectively, using spectroscopic methods (UV-Visand MS). The three anthocyanins were isolated and purified by preparative HPLC, and their chemical structures were further characterised by H1 NMR. On the basis of chromatographic data, the total anthocyanin content was 286 mg/g in fresh fruit of waxberry. |
| first_indexed | 2024-04-10T08:35:21Z |
| format | Article |
| id | doaj.art-db958dbf3b4b4b4b89c1533347c62606 |
| institution | Directory Open Access Journal |
| issn | 1212-1800 1805-9317 |
| language | English |
| last_indexed | 2024-04-10T08:35:21Z |
| publishDate | 2011-04-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Czech Journal of Food Sciences |
| spelling | doaj.art-db958dbf3b4b4b4b89c1533347c626062023-02-23T03:27:39ZengCzech Academy of Agricultural SciencesCzech Journal of Food Sciences1212-18001805-93172011-04-0129217118010.17221/177/2010-CJFScjf-201102-0010Composition analysis and structural identification of anthocyanins in fruit of waxberryChuan-Guang Qin0Yang Li1Weining Niu2Yan Ding3Xiaoya Shang4Chunlan Xu5Faculty of Life Science, Northwestern Polytechnical University, Xi'an, Shaanxi, P. R. ChinaFaculty of Life Science, Northwestern Polytechnical University, Xi'an, Shaanxi, P. R. ChinaFaculty of Life Science, Northwestern Polytechnical University, Xi'an, Shaanxi, P. R. ChinaFaculty of Life Science, Northwestern Polytechnical University, Xi'an, Shaanxi, P. R. ChinaFaculty of Life Science, Northwestern Polytechnical University, Xi'an, Shaanxi, P. R. ChinaFaculty of Life Science, Northwestern Polytechnical University, Xi'an, Shaanxi, P. R. ChinaAnthocyanin pigments in the fruit of waxberry (Myrica rubra Sieb. et Zucc.), were extracted with 0.1% HCl in ethanol, and the crude anthocyanin extract was purified by C18 Sep-Pak cartridge open-column chromatography. High-performance liquid chromatography (HPLC) with photodiode array detection (PAD) and matrix-assisted laser desorption/ionisation-time of flight-mass spectrometry (MALDI-TOF-MS) was applied for the separation and identification of anthocyanins in the fruit of waxberry and their aglycones resulting from acid hydrolysis. Three anthocyanins were found in the fruit of waxberry and identified as Cyanidin 3-O-β-galacopyranoside (14.8%), Cyanidin 3-O-β-gluco-pyranoside (60.5%), and petunidin 3-O-β-glucopyranoside (24.7%), respectively, using spectroscopic methods (UV-Visand MS). The three anthocyanins were isolated and purified by preparative HPLC, and their chemical structures were further characterised by H1 NMR. On the basis of chromatographic data, the total anthocyanin content was 286 mg/g in fresh fruit of waxberry.https://cjfs.agriculturejournals.cz/artkey/cjf-201102-0010_composition-analysis-and-structural-identification-of-anthocyanins-in-fruit-of-waxberry.phpwaxberry (myrica rubra sieb. et zucc.)anthocyaninnatural productpigment identificationcomposition analysis |
| spellingShingle | Chuan-Guang Qin Yang Li Weining Niu Yan Ding Xiaoya Shang Chunlan Xu Composition analysis and structural identification of anthocyanins in fruit of waxberry Czech Journal of Food Sciences waxberry (myrica rubra sieb. et zucc.) anthocyanin natural product pigment identification composition analysis |
| title | Composition analysis and structural identification of anthocyanins in fruit of waxberry |
| title_full | Composition analysis and structural identification of anthocyanins in fruit of waxberry |
| title_fullStr | Composition analysis and structural identification of anthocyanins in fruit of waxberry |
| title_full_unstemmed | Composition analysis and structural identification of anthocyanins in fruit of waxberry |
| title_short | Composition analysis and structural identification of anthocyanins in fruit of waxberry |
| title_sort | composition analysis and structural identification of anthocyanins in fruit of waxberry |
| topic | waxberry (myrica rubra sieb. et zucc.) anthocyanin natural product pigment identification composition analysis |
| url | https://cjfs.agriculturejournals.cz/artkey/cjf-201102-0010_composition-analysis-and-structural-identification-of-anthocyanins-in-fruit-of-waxberry.php |
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