A Rapid Assay to Detect Toxigenic Penicillium spp. Contamination in Wine and Musts
Wine and fermenting musts are grape products widely consumed worldwide. Since the presence of mycotoxin-producing fungi may greatly compromise their quality characteristics and safety, there is an increasing need for relatively rapid “user friendly” quantitative assays to detect fungal contamination...
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| Format: | Article |
| Language: | English |
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MDPI AG
2016-08-01
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| Series: | Toxins |
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| Online Access: | http://www.mdpi.com/2072-6651/8/8/235 |
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| author | Simona Marianna Sanzani Monica Marilena Miazzi Valentina di Rienzo Valentina Fanelli Giuseppe Gambacorta Maria Rosaria Taurino Cinzia Montemurro |
| author_facet | Simona Marianna Sanzani Monica Marilena Miazzi Valentina di Rienzo Valentina Fanelli Giuseppe Gambacorta Maria Rosaria Taurino Cinzia Montemurro |
| author_sort | Simona Marianna Sanzani |
| collection | DOAJ |
| description | Wine and fermenting musts are grape products widely consumed worldwide. Since the presence of mycotoxin-producing fungi may greatly compromise their quality characteristics and safety, there is an increasing need for relatively rapid “user friendly” quantitative assays to detect fungal contamination both in grapes delivered to wineries and in final products. Although other fungi are most frequently involved in grape deterioration, secondary infections by Penicillium spp. are quite common, especially in cool areas with high humidity and in wines obtained by partially dried grapes. In this work, a single-tube nested real-time PCR approach—successfully applied to hazelnut and peanut allergen detection—was tested for the first time to trace Penicillium spp. in musts and wines. The method consisted of two sets of primers specifically designed to target the β-tubulin gene, to be simultaneously applied with the aim of lowering the detection limit of conventional real-time PCR. The assay was able to detect up to 1 fg of Penicillium DNA. As confirmation, patulin content of representative samples was determined. Most of analyzed wines/musts returned contaminated results at >50 ppb and a 76% accordance with molecular assay was observed. Although further large-scale trials are needed, these results encourage the use of the newly developed method in the pre-screening of fresh and processed grapes for the presence of Penicillium DNA before the evaluation of related toxins. |
| first_indexed | 2024-04-14T00:51:12Z |
| format | Article |
| id | doaj.art-dbf67a8a544f4f08a7998e9fd393c7b2 |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-04-14T00:51:12Z |
| publishDate | 2016-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-dbf67a8a544f4f08a7998e9fd393c7b22022-12-22T02:21:47ZengMDPI AGToxins2072-66512016-08-018823510.3390/toxins8080235toxins8080235A Rapid Assay to Detect Toxigenic Penicillium spp. Contamination in Wine and MustsSimona Marianna Sanzani0Monica Marilena Miazzi1Valentina di Rienzo2Valentina Fanelli3Giuseppe Gambacorta4Maria Rosaria Taurino5Cinzia Montemurro6Dipartimento di Scienze del Suolo, della Pianta e degli Alimenti, Università degli Studi di Bari Aldo Moro, Via G. Amendola 165/A, 70126 Bari, ItalyDipartimento di Scienze del Suolo, della Pianta e degli Alimenti, Università degli Studi di Bari Aldo Moro, Via G. Amendola 165/A, 70126 Bari, ItalyDipartimento di Scienze del Suolo, della Pianta e degli Alimenti, Università degli Studi di Bari Aldo Moro, Via G. Amendola 165/A, 70126 Bari, ItalyDipartimento di Scienze del Suolo, della Pianta e degli Alimenti, Università degli Studi di Bari Aldo Moro, Via G. Amendola 165/A, 70126 Bari, ItalyDipartimento di Scienze del Suolo, della Pianta e degli Alimenti, Università degli Studi di Bari Aldo Moro, Via G. Amendola 165/A, 70126 Bari, ItalyAgro.Biolab Laboratory s.r.l., SP240 km 13,800, 70018 Rutigliano (BA), ItalyDipartimento di Scienze del Suolo, della Pianta e degli Alimenti, Università degli Studi di Bari Aldo Moro, Via G. Amendola 165/A, 70126 Bari, ItalyWine and fermenting musts are grape products widely consumed worldwide. Since the presence of mycotoxin-producing fungi may greatly compromise their quality characteristics and safety, there is an increasing need for relatively rapid “user friendly” quantitative assays to detect fungal contamination both in grapes delivered to wineries and in final products. Although other fungi are most frequently involved in grape deterioration, secondary infections by Penicillium spp. are quite common, especially in cool areas with high humidity and in wines obtained by partially dried grapes. In this work, a single-tube nested real-time PCR approach—successfully applied to hazelnut and peanut allergen detection—was tested for the first time to trace Penicillium spp. in musts and wines. The method consisted of two sets of primers specifically designed to target the β-tubulin gene, to be simultaneously applied with the aim of lowering the detection limit of conventional real-time PCR. The assay was able to detect up to 1 fg of Penicillium DNA. As confirmation, patulin content of representative samples was determined. Most of analyzed wines/musts returned contaminated results at >50 ppb and a 76% accordance with molecular assay was observed. Although further large-scale trials are needed, these results encourage the use of the newly developed method in the pre-screening of fresh and processed grapes for the presence of Penicillium DNA before the evaluation of related toxins.http://www.mdpi.com/2072-6651/8/8/235grapePenicillium detectionmycotoxinsfood safety |
| spellingShingle | Simona Marianna Sanzani Monica Marilena Miazzi Valentina di Rienzo Valentina Fanelli Giuseppe Gambacorta Maria Rosaria Taurino Cinzia Montemurro A Rapid Assay to Detect Toxigenic Penicillium spp. Contamination in Wine and Musts Toxins grape Penicillium detection mycotoxins food safety |
| title | A Rapid Assay to Detect Toxigenic Penicillium spp. Contamination in Wine and Musts |
| title_full | A Rapid Assay to Detect Toxigenic Penicillium spp. Contamination in Wine and Musts |
| title_fullStr | A Rapid Assay to Detect Toxigenic Penicillium spp. Contamination in Wine and Musts |
| title_full_unstemmed | A Rapid Assay to Detect Toxigenic Penicillium spp. Contamination in Wine and Musts |
| title_short | A Rapid Assay to Detect Toxigenic Penicillium spp. Contamination in Wine and Musts |
| title_sort | rapid assay to detect toxigenic penicillium spp contamination in wine and musts |
| topic | grape Penicillium detection mycotoxins food safety |
| url | http://www.mdpi.com/2072-6651/8/8/235 |
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