Encapsulating IM7-Displaying Yeast Cells in Calcium Alginate Beads for One-Step Protein Purification and Multienzyme Biocatalysis

Encapsulating IM7-Displaying Yeast Cells in Calcium Alginate Beads for One-Step Protein Purification and Multienzyme Biocatalysis

There are several commercial chromatographic systems for protein purification; however, development of cost-effective 3H-grade (high yield, high purity, and high activity) purification approaches is highly demanded. Here, we establish a methodology for encapsulating the IM7-displaying yeast cells in...

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Bibliographic Details
Main Authors: Wenhao Yin, Xinping Wang, Ying Liao, Lixin Ma, Jie Qiao, Hui Liu, Xin Song, Yi Liu
Format: Article
Language:English
Published: Frontiers Media S.A. 2022-03-01
Series:Frontiers in Bioengineering and Biotechnology
Subjects:
Colicin E7/IM7 affinity chromatography
one-step protein purification
multiple-enzyme complex
biocatalysis
enzyme immobilization
Online Access:https://www.frontiersin.org/articles/10.3389/fbioe.2022.849542/full

Internet

https://www.frontiersin.org/articles/10.3389/fbioe.2022.849542/full

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