Rich dynamics and functional organization on topographically designed neuronal networks in vitro
Summary: Neuronal cultures are a prominent experimental tool to understand complex functional organization in neuronal assemblies. However, neurons grown on flat surfaces exhibit a strongly coherent bursting behavior with limited functionality. To approach the functional richness of naturally formed...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-12-01
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| Series: | iScience |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004222019526 |
| _version_ | 1798006200856477696 |
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| author | Marc Montalà-Flaquer Clara F. López-León Daniel Tornero Akke Mats Houben Tanguy Fardet Pascal Monceau Samuel Bottani Jordi Soriano |
| author_facet | Marc Montalà-Flaquer Clara F. López-León Daniel Tornero Akke Mats Houben Tanguy Fardet Pascal Monceau Samuel Bottani Jordi Soriano |
| author_sort | Marc Montalà-Flaquer |
| collection | DOAJ |
| description | Summary: Neuronal cultures are a prominent experimental tool to understand complex functional organization in neuronal assemblies. However, neurons grown on flat surfaces exhibit a strongly coherent bursting behavior with limited functionality. To approach the functional richness of naturally formed neuronal circuits, here we studied neuronal networks grown on polydimethylsiloxane (PDMS) topographical patterns shaped as either parallel tracks or square valleys. We followed the evolution of spontaneous activity in these cultures along 20 days in vitro using fluorescence calcium imaging. The networks were characterized by rich spatiotemporal activity patterns that comprised from small regions of the culture to its whole extent. Effective connectivity analysis revealed the emergence of spatially compact functional modules that were associated with both the underpinned topographical features and predominant spatiotemporal activity fronts. Our results show the capacity of spatial constraints to mold activity and functional organization, bringing new opportunities to comprehend the structure-function relationship in living neuronal circuits. |
| first_indexed | 2024-04-11T12:52:18Z |
| format | Article |
| id | doaj.art-dc1c5d87702f4a6e985c5632b95ad74d |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-04-11T12:52:18Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-dc1c5d87702f4a6e985c5632b95ad74d2022-12-22T04:23:11ZengElsevieriScience2589-00422022-12-012512105680Rich dynamics and functional organization on topographically designed neuronal networks in vitroMarc Montalà-Flaquer0Clara F. López-León1Daniel Tornero2Akke Mats Houben3Tanguy Fardet4Pascal Monceau5Samuel Bottani6Jordi Soriano7Departament de Física de la Matèria Condensada, Universitat de Barcelona, E-08028 Barcelona, Spain; Universitat de Barcelona Institute of Complex Systems (UBICS), E-08028 Barcelona, SpainDepartament de Física de la Matèria Condensada, Universitat de Barcelona, E-08028 Barcelona, Spain; Universitat de Barcelona Institute of Complex Systems (UBICS), E-08028 Barcelona, SpainLaboratory of Neural Stem Cells and Brain Damage, Institute of Neurosciences, University of Barcelona, E-08036 Barcelona, SpainDepartament de Física de la Matèria Condensada, Universitat de Barcelona, E-08028 Barcelona, Spain; Universitat de Barcelona Institute of Complex Systems (UBICS), E-08028 Barcelona, SpainLaboratoire Matière et Systèmes Complexes, Université de Paris, UMR 7057 CNRS, Paris, France; University of Tübingen, Tübingen, Germany; Max Planck Institute for Biological Cybernetics, Tübingen, GermanyLaboratoire Matière et Systèmes Complexes, Université de Paris, UMR 7057 CNRS, Paris, FranceLaboratoire Matière et Systèmes Complexes, Université de Paris, UMR 7057 CNRS, Paris, FranceDepartament de Física de la Matèria Condensada, Universitat de Barcelona, E-08028 Barcelona, Spain; Universitat de Barcelona Institute of Complex Systems (UBICS), E-08028 Barcelona, Spain; Corresponding authorSummary: Neuronal cultures are a prominent experimental tool to understand complex functional organization in neuronal assemblies. However, neurons grown on flat surfaces exhibit a strongly coherent bursting behavior with limited functionality. To approach the functional richness of naturally formed neuronal circuits, here we studied neuronal networks grown on polydimethylsiloxane (PDMS) topographical patterns shaped as either parallel tracks or square valleys. We followed the evolution of spontaneous activity in these cultures along 20 days in vitro using fluorescence calcium imaging. The networks were characterized by rich spatiotemporal activity patterns that comprised from small regions of the culture to its whole extent. Effective connectivity analysis revealed the emergence of spatially compact functional modules that were associated with both the underpinned topographical features and predominant spatiotemporal activity fronts. Our results show the capacity of spatial constraints to mold activity and functional organization, bringing new opportunities to comprehend the structure-function relationship in living neuronal circuits.http://www.sciencedirect.com/science/article/pii/S2589004222019526NeuroscienceCell biologyNeural networks |
| spellingShingle | Marc Montalà-Flaquer Clara F. López-León Daniel Tornero Akke Mats Houben Tanguy Fardet Pascal Monceau Samuel Bottani Jordi Soriano Rich dynamics and functional organization on topographically designed neuronal networks in vitro iScience Neuroscience Cell biology Neural networks |
| title | Rich dynamics and functional organization on topographically designed neuronal networks in vitro |
| title_full | Rich dynamics and functional organization on topographically designed neuronal networks in vitro |
| title_fullStr | Rich dynamics and functional organization on topographically designed neuronal networks in vitro |
| title_full_unstemmed | Rich dynamics and functional organization on topographically designed neuronal networks in vitro |
| title_short | Rich dynamics and functional organization on topographically designed neuronal networks in vitro |
| title_sort | rich dynamics and functional organization on topographically designed neuronal networks in vitro |
| topic | Neuroscience Cell biology Neural networks |
| url | http://www.sciencedirect.com/science/article/pii/S2589004222019526 |
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