Regulation of hypoxia-inducible factor 1α and induction of vascular endothelial growth factor in a rat neonatal stroke model
Stroke is a devastating condition occurring in at least 1 in 4000 live births in the neonatal period. Since hypoxia-inducible factor (HIF)-1α can modulate ischemic injury via induction of target genes that may protect cells against ischemia, and is induced after preconditioning by hypoxia in the neo...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2003-12-01
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| Series: | Neurobiology of Disease |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0969996103001694 |
| _version_ | 1830138859926585344 |
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| author | Dezhi Mu Xiangning Jiang R.Ann Sheldon Christine K Fox Shannon E.G Hamrick Zinaida S Vexler Donna M Ferriero |
| author_facet | Dezhi Mu Xiangning Jiang R.Ann Sheldon Christine K Fox Shannon E.G Hamrick Zinaida S Vexler Donna M Ferriero |
| author_sort | Dezhi Mu |
| collection | DOAJ |
| description | Stroke is a devastating condition occurring in at least 1 in 4000 live births in the neonatal period. Since hypoxia-inducible factor (HIF)-1α can modulate ischemic injury via induction of target genes that may protect cells against ischemia, and is induced after preconditioning by hypoxia in the neonatal rat brain hypoxia-ischemia model, we evaluated whether HIF-1α is induced after focal ischemia-reperfusion, a model for neonatal stroke. We developed an ischemia-reperfusion model in postnatal day 10 (P10) rats by transiently occluding the middle cerebral artery (MCA) for 1.5 h. The MCA territory was reperfused for 0, 4, 8, or 24 h and the expression of HIF-1α and its target gene, vascular endothelial growth factor (VEGF), were delineated. HIF-1α protein and VEGF protein peaked at 8 h, and declined subsequently at 24 h in injured cortex following 1.5 h of MCA occlusion. Double-immunolabeling indicated that both HIF-1α and VEGF are expressed together in neurons with a similar time course of expression. The presence of HIF-1α and VEGF after moderate ischemia-reperfusion injury suggests potential avenues to exploit for neuroprotection. |
| first_indexed | 2024-12-17T08:33:39Z |
| format | Article |
| id | doaj.art-dc1d3f55e6cf425697bd3390aef75abe |
| institution | Directory Open Access Journal |
| issn | 1095-953X |
| language | English |
| last_indexed | 2024-12-17T08:33:39Z |
| publishDate | 2003-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Neurobiology of Disease |
| spelling | doaj.art-dc1d3f55e6cf425697bd3390aef75abe2022-12-21T21:56:32ZengElsevierNeurobiology of Disease1095-953X2003-12-01143524534Regulation of hypoxia-inducible factor 1α and induction of vascular endothelial growth factor in a rat neonatal stroke modelDezhi Mu0Xiangning Jiang1R.Ann Sheldon2Christine K Fox3Shannon E.G Hamrick4Zinaida S Vexler5Donna M Ferriero6Department of Neurology, University of California San Francisco, San Francisco, CA, USA; Department of Pediatrics, University of California San Francisco, San Francisco, CA, USADepartment of Neurology, University of California San Francisco, San Francisco, CA, USA; Department of Pediatrics, University of California San Francisco, San Francisco, CA, USADepartment of Neurology, University of California San Francisco, San Francisco, CA, USA; Department of Pediatrics, University of California San Francisco, San Francisco, CA, USADepartment of Neurology, University of California San Francisco, San Francisco, CA, USA; Department of Pediatrics, University of California San Francisco, San Francisco, CA, USADepartment of Neurology, University of California San Francisco, San Francisco, CA, USA; Department of Pediatrics, University of California San Francisco, San Francisco, CA, USADepartment of Neurology, University of California San Francisco, San Francisco, CA, USA; Department of Pediatrics, University of California San Francisco, San Francisco, CA, USADepartment of Neurology, University of California San Francisco, San Francisco, CA, USA; Department of Pediatrics, University of California San Francisco, San Francisco, CA, USAStroke is a devastating condition occurring in at least 1 in 4000 live births in the neonatal period. Since hypoxia-inducible factor (HIF)-1α can modulate ischemic injury via induction of target genes that may protect cells against ischemia, and is induced after preconditioning by hypoxia in the neonatal rat brain hypoxia-ischemia model, we evaluated whether HIF-1α is induced after focal ischemia-reperfusion, a model for neonatal stroke. We developed an ischemia-reperfusion model in postnatal day 10 (P10) rats by transiently occluding the middle cerebral artery (MCA) for 1.5 h. The MCA territory was reperfused for 0, 4, 8, or 24 h and the expression of HIF-1α and its target gene, vascular endothelial growth factor (VEGF), were delineated. HIF-1α protein and VEGF protein peaked at 8 h, and declined subsequently at 24 h in injured cortex following 1.5 h of MCA occlusion. Double-immunolabeling indicated that both HIF-1α and VEGF are expressed together in neurons with a similar time course of expression. The presence of HIF-1α and VEGF after moderate ischemia-reperfusion injury suggests potential avenues to exploit for neuroprotection.http://www.sciencedirect.com/science/article/pii/S0969996103001694DevelopmentIschemiaBrainToleranceNeuroprotection |
| spellingShingle | Dezhi Mu Xiangning Jiang R.Ann Sheldon Christine K Fox Shannon E.G Hamrick Zinaida S Vexler Donna M Ferriero Regulation of hypoxia-inducible factor 1α and induction of vascular endothelial growth factor in a rat neonatal stroke model Neurobiology of Disease Development Ischemia Brain Tolerance Neuroprotection |
| title | Regulation of hypoxia-inducible factor 1α and induction of vascular endothelial growth factor in a rat neonatal stroke model |
| title_full | Regulation of hypoxia-inducible factor 1α and induction of vascular endothelial growth factor in a rat neonatal stroke model |
| title_fullStr | Regulation of hypoxia-inducible factor 1α and induction of vascular endothelial growth factor in a rat neonatal stroke model |
| title_full_unstemmed | Regulation of hypoxia-inducible factor 1α and induction of vascular endothelial growth factor in a rat neonatal stroke model |
| title_short | Regulation of hypoxia-inducible factor 1α and induction of vascular endothelial growth factor in a rat neonatal stroke model |
| title_sort | regulation of hypoxia inducible factor 1α and induction of vascular endothelial growth factor in a rat neonatal stroke model |
| topic | Development Ischemia Brain Tolerance Neuroprotection |
| url | http://www.sciencedirect.com/science/article/pii/S0969996103001694 |
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