Structures of apo Cas12a and its complex with crRNA and DNA reveal the dynamics of ternary complex formation and target DNA cleavage.

Structures of apo Cas12a and its complex with crRNA and DNA reveal the dynamics of ternary complex formation and target DNA cleavage.

Cas12a is a programmable nuclease for adaptive immunity against invading nucleic acids in CRISPR-Cas systems. Here, we report the crystal structures of apo Cas12a from Lachnospiraceae bacterium MA2020 (Lb2) and the Lb2Cas12a+crRNA complex, as well as the cryo-EM structure and functional studies of t...

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Bibliographic Details
Main Authors: Li Jianwei, Chacko Jobichen, Satoru Machida, Sun Meng, Randy J Read, Chen Hongying, Shi Jian, Yuren Adam Yuan, J Sivaraman
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2023-03-01
Series:PLoS Biology
Online Access:https://doi.org/10.1371/journal.pbio.3002023

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https://doi.org/10.1371/journal.pbio.3002023

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