An integrated workflow for crosslinking mass spectrometry

An integrated workflow for crosslinking mass spectrometry

Abstract We present a concise workflow to enhance the mass spectrometric detection of crosslinked peptides by introducing sequential digestion and the crosslink identification software xiSEARCH. Sequential digestion enhances peptide detection by selective shortening of long tryptic peptides. We demo...

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Bibliographic Details
Main Authors: Marta L Mendes, Lutz Fischer, Zhuo A Chen, Marta Barbon, Francis J O'Reilly, Sven H Giese, Michael Bohlke‐Schneider, Adam Belsom, Therese Dau, Colin W Combe, Martin Graham, Markus R Eisele, Wolfgang Baumeister, Christian Speck, Juri Rappsilber
Format: Article
Language:English
Published: Springer Nature 2019-09-01
Series:Molecular Systems Biology
Subjects:
crosslinking mass spectrometry
protein–protein interactions
proteomics
software
structural biology
Online Access:https://doi.org/10.15252/msb.20198994

Internet

https://doi.org/10.15252/msb.20198994

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