Separation of spectrally overlapping fluorophores using intra-exposure excitation modulation

Multicolor fluorescence imaging is an excellent method for the simultaneous visualization of multiple structures, although it is limited by the available spectral window. More labels can be measured by distinguishing these on properties, such as their fluorescence dynamics, but usually these dynamic...

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Bibliographic Details
Main Authors: Hana Valenta, Siewert Hugelier, Sam Duwé, Giulia Lo Gerfo, Marcel Müller, Peter Dedecker, Wim Vandenberg
Format: Article
Language:English
Published: Elsevier 2021-12-01
Series:Biophysical Reports
Online Access:http://www.sciencedirect.com/science/article/pii/S2667074721000264