Engineering an adenine base editor in human embryonic stem cells with minimal DNA and RNA off-target activities

Engineering an adenine base editor in human embryonic stem cells with minimal DNA and RNA off-target activities

Genome editing in pluripotent stem cells (PSCs) using CRISPR technology holds great promise for therapeutic applications. Yet, it has been reported that Cas9-mediated cleavage could cause large deletions or rearrangements of DNA, and the selection of edited PSCs could acquire p53 mutations. Adenine...

Full description

Bibliographic Details
Main Authors: Zhenwu Zhang, Wanyu Tao, Shisheng Huang, Wenjun Sun, Yue Wang, Wen Jiang, Xingxu Huang, Chao-Po Lin
Format: Article
Language:English
Published: Elsevier 2022-09-01
Series:Molecular Therapy: Nucleic Acids
Subjects:
MT: RNA/DNA Editing
CRISPR
off-target effect
adenine base editor
human embryonic stem cell
Online Access:http://www.sciencedirect.com/science/article/pii/S2162253122001950

Internet

http://www.sciencedirect.com/science/article/pii/S2162253122001950

Similar Items