Liquid Chromatography-Tandem Mass Spectrometry Analysis of Acetaminophen Covalent Binding to Glutathione S-Transferases

Liquid Chromatography-Tandem Mass Spectrometry Analysis of Acetaminophen Covalent Binding to Glutathione S-Transferases

Acetaminophen (APAP)-induced hepatotoxicity is the most common cause of acute liver failure in the Western world. APAP is bioactivated to N-acetyl p-benzoquinone imine (NAPQI), a reactive metabolite, which can subsequently covalently bind to glutathione and protein thiols. In this study, we have use...

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Bibliographic Details
Main Authors: Timon Geib, Cristina Lento, Derek J. Wilson, Lekha Sleno
Format: Article
Language:English
Published: Frontiers Media S.A. 2019-08-01
Series:Frontiers in Chemistry
Subjects:
acetaminophen
reactive metabolite
covalent binding
glutathione S-transferase
bottom-up proteomics
data-dependent acquisition
Online Access:https://www.frontiersin.org/article/10.3389/fchem.2019.00558/full

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https://www.frontiersin.org/article/10.3389/fchem.2019.00558/full

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