Enzymatic Activity Assays for Base Excision Repair Enzymes in Cell Extracts from Vertebrate Cells

We previously reported enzymatic activity assays for the base excision repair (BER) enzymes DNA polymerase β (pol β), aprataxin (APTX), and flap endonuclease 1 (FEN1) in cell extracts from Saccharomyces cerevisiae (Çağlayan and Wilson, 2014). Here, we describe a method to prepare cell extracts from...

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Bibliographic Details
Main Authors: Melike Çağlayan, Julie Horton, Samuel Wilson
Format: Article
Language:English
Published: Bio-protocol LLC 2015-06-01
Series:Bio-Protocol
Online Access:https://bio-protocol.org/en/bpdetail?id=1493&type=0