How CD4<sup>+</sup> T Cells Transcriptional Profile Is Affected by Culture Conditions: Towards the Design of Optimal In Vitro HIV Reactivation Assays

How CD4<sup>+</sup> T Cells Transcriptional Profile Is Affected by Culture Conditions: Towards the Design of Optimal In Vitro HIV Reactivation Assays

Most of the current assays directed at the investigation of HIV reactivation are based on cultures of infected cells such as Peripheral Blood Mononuclear Cells (PBMCs) or isolated CD4<sup>+</sup> T cells, stimulated in vitro with different activator molecules. The culture media in these...

Full description

Bibliographic Details
Main Authors: Giuseppe Rubens Pascucci, Elena Morrocchi, Chiara Pighi, Arianna Rotili, Alessia Neri, Chiara Medri, Giulio Olivieri, Marco Sanna, Gianmarco Rasi, Deborah Persaud, Ann Chahroudi, Mathias Lichterfeld, Eleni Nastouli, Caterina Cancrini, Donato Amodio, Paolo Rossi, Nicola Cotugno, Paolo Palma
Format: Article
Language:English
Published: MDPI AG 2023-03-01
Series:Biomedicines
Subjects:
T cell activation
in vitro cultures
HIV reactivation
RNA sequencing
Oxford Nanopore technologies
autologous plasma
Online Access:https://www.mdpi.com/2227-9059/11/3/888

Internet

https://www.mdpi.com/2227-9059/11/3/888

Similar Items