Non-flipping DNA glycosylase AlkD scans DNA without formation of a stable interrogation complex
Ahmadi et al. use a single-molecule tracking method to describe the DNA scanning mode of AlkD, a HEAT-like repeat DNA glycosylase. They show that, contrary to other glycosylases that use a base-flipping mechanism, AlkD scans the DNA without forming a stable interrogation complex.
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2021-07-01
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| Series: | Communications Biology |
| Online Access: | https://doi.org/10.1038/s42003-021-02400-x |
| _version_ | 1818750733185449984 |
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| author | Arash Ahmadi Katharina Till Paul Hoff Backe Pernille Blicher Robin Diekmann Mark Schüttpelz Kyrre Glette Jim Tørresen Magnar Bjørås Alexander D. Rowe Bjørn Dalhus |
| author_facet | Arash Ahmadi Katharina Till Paul Hoff Backe Pernille Blicher Robin Diekmann Mark Schüttpelz Kyrre Glette Jim Tørresen Magnar Bjørås Alexander D. Rowe Bjørn Dalhus |
| author_sort | Arash Ahmadi |
| collection | DOAJ |
| description | Ahmadi et al. use a single-molecule tracking method to describe the DNA scanning mode of AlkD, a HEAT-like repeat DNA glycosylase. They show that, contrary to other glycosylases that use a base-flipping mechanism, AlkD scans the DNA without forming a stable interrogation complex. |
| first_indexed | 2024-12-18T04:24:22Z |
| format | Article |
| id | doaj.art-dc979ac48443432c9defb5314d98f0c6 |
| institution | Directory Open Access Journal |
| issn | 2399-3642 |
| language | English |
| last_indexed | 2024-12-18T04:24:22Z |
| publishDate | 2021-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Communications Biology |
| spelling | doaj.art-dc979ac48443432c9defb5314d98f0c62022-12-21T21:21:10ZengNature PortfolioCommunications Biology2399-36422021-07-01411810.1038/s42003-021-02400-xNon-flipping DNA glycosylase AlkD scans DNA without formation of a stable interrogation complexArash Ahmadi0Katharina Till1Paul Hoff Backe2Pernille Blicher3Robin Diekmann4Mark Schüttpelz5Kyrre Glette6Jim Tørresen7Magnar Bjørås8Alexander D. Rowe9Bjørn Dalhus10Department of Medical Biochemistry, Institute for Clinical Medicine, University of OsloFOM Institute AMOLF, Science Park 104Department of Medical Biochemistry, Institute for Clinical Medicine, University of OsloDepartment of Medical Biochemistry, Institute for Clinical Medicine, University of OsloBiomolecular Photonics, Department of Physics, University of BielefeldBiomolecular Photonics, Department of Physics, University of BielefeldDepartment of Informatics, University of OsloDepartment of Informatics, University of OsloDepartment of Microbiology, Oslo University Hospital HF, Rikshospitalet and University of OsloDepartment of Medical Biochemistry, Institute for Clinical Medicine, University of OsloDepartment of Medical Biochemistry, Institute for Clinical Medicine, University of OsloAhmadi et al. use a single-molecule tracking method to describe the DNA scanning mode of AlkD, a HEAT-like repeat DNA glycosylase. They show that, contrary to other glycosylases that use a base-flipping mechanism, AlkD scans the DNA without forming a stable interrogation complex.https://doi.org/10.1038/s42003-021-02400-x |
| spellingShingle | Arash Ahmadi Katharina Till Paul Hoff Backe Pernille Blicher Robin Diekmann Mark Schüttpelz Kyrre Glette Jim Tørresen Magnar Bjørås Alexander D. Rowe Bjørn Dalhus Non-flipping DNA glycosylase AlkD scans DNA without formation of a stable interrogation complex Communications Biology |
| title | Non-flipping DNA glycosylase AlkD scans DNA without formation of a stable interrogation complex |
| title_full | Non-flipping DNA glycosylase AlkD scans DNA without formation of a stable interrogation complex |
| title_fullStr | Non-flipping DNA glycosylase AlkD scans DNA without formation of a stable interrogation complex |
| title_full_unstemmed | Non-flipping DNA glycosylase AlkD scans DNA without formation of a stable interrogation complex |
| title_short | Non-flipping DNA glycosylase AlkD scans DNA without formation of a stable interrogation complex |
| title_sort | non flipping dna glycosylase alkd scans dna without formation of a stable interrogation complex |
| url | https://doi.org/10.1038/s42003-021-02400-x |
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