Establishment of PIN1 knockout porcine fetal fibroblast cell lines via CRISPR/Cas9 mediated gene-targeting

Establishment of PIN1 knockout porcine fetal fibroblast cell lines via CRISPR/Cas9 mediated gene-targeting

Objective To knock out PIN1 gene of porcine fetal fibroblasts (PFFs) by clustered regular interval short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) system in order to establish PIN1 knockout porcine fetal fibroblast cell lines. Methods Firstly, the homology of the PIN1 gene betw...

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Bibliographic Details
Main Authors: WANG Wanyi, YUAN Yilin, LI Lin, WANG Ying, DAI Yifan, YANG Haiyuan
Format: Article
Language:zho
Published: Editorial Office of Journal of Army Medical University 2022-07-01
Series:陆军军医大学学报
Subjects:
crispr/cas9
pin1
porcine fetal fibroblasts
pig disease model
Online Access:http://aammt.tmmu.edu.cn/Upload/rhtml/202112217.htm

Internet

http://aammt.tmmu.edu.cn/Upload/rhtml/202112217.htm

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