Determination of 9 yellow colorants in fish tissues by ultra performance liquid chromatography-tandem mass spectrometry
ObjectiveTo establish a detection method for 9 yellow colorants in fish tissues by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS).MethodsSample was extracted by ammonia methanol solution and formic acid water methanol solution, the supernatant was evaporated and rediss...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | zho |
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The Editorial Office of Chinese Journal of Food Hygiene
2022-01-01
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| Series: | Zhongguo shipin weisheng zazhi |
| Subjects: | |
| Online Access: | http://www.zgspws.com/zgspwszz/article/abstract/20220109?st=article_issue |
| _version_ | 1828085964552011776 |
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| author | PAN Ying LV Shenliang XIA Sujie CHEN Yan XU Weidong |
| author_facet | PAN Ying LV Shenliang XIA Sujie CHEN Yan XU Weidong |
| author_sort | PAN Ying |
| collection | DOAJ |
| description | ObjectiveTo establish a detection method for 9 yellow colorants in fish tissues by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS).MethodsSample was extracted by ammonia methanol solution and formic acid water methanol solution, the supernatant was evaporated and redissolved, the fat was removed by n-hexane, determinated by UPLC-MS/MS method after centrifugation, and quantification was based on external calibration.ResultsThe result showed good linearity in the ranges of 2-30 μg/kg for tartrazine, sunset yellow and quinoline yellow, 0.2-3.0 μg/kg for acid orange Ⅱ, basic orange 2, basic orange 21, basic orange 22, thioflavin T and auramine O, and the limits of detection were 2 μg/kg and 0.2 μg/kg. The recoveries were 80.03%-107.06% and the relative standard deviations(n=6)were 1.2%-6.6%.ConclusionThe method can be applied to determine 9 yellow colorants in fish tissues. |
| first_indexed | 2024-04-11T04:48:15Z |
| format | Article |
| id | doaj.art-dcd82ef25c07416d94d01fef297ed78d |
| institution | Directory Open Access Journal |
| issn | 1004-8456 |
| language | zho |
| last_indexed | 2024-04-11T04:48:15Z |
| publishDate | 2022-01-01 |
| publisher | The Editorial Office of Chinese Journal of Food Hygiene |
| record_format | Article |
| series | Zhongguo shipin weisheng zazhi |
| spelling | doaj.art-dcd82ef25c07416d94d01fef297ed78d2022-12-27T09:24:30ZzhoThe Editorial Office of Chinese Journal of Food HygieneZhongguo shipin weisheng zazhi1004-84562022-01-013401444810.13590/j.cjfh.2022.01.0091004-8456(2022)01-0044-05Determination of 9 yellow colorants in fish tissues by ultra performance liquid chromatography-tandem mass spectrometryPAN YingLV ShenliangXIA SujieCHEN YanXU WeidongObjectiveTo establish a detection method for 9 yellow colorants in fish tissues by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS).MethodsSample was extracted by ammonia methanol solution and formic acid water methanol solution, the supernatant was evaporated and redissolved, the fat was removed by n-hexane, determinated by UPLC-MS/MS method after centrifugation, and quantification was based on external calibration.ResultsThe result showed good linearity in the ranges of 2-30 μg/kg for tartrazine, sunset yellow and quinoline yellow, 0.2-3.0 μg/kg for acid orange Ⅱ, basic orange 2, basic orange 21, basic orange 22, thioflavin T and auramine O, and the limits of detection were 2 μg/kg and 0.2 μg/kg. The recoveries were 80.03%-107.06% and the relative standard deviations(n=6)were 1.2%-6.6%.ConclusionThe method can be applied to determine 9 yellow colorants in fish tissues.http://www.zgspws.com/zgspwszz/article/abstract/20220109?st=article_issuefish tissuesyellow colorantsultra high-performance liquid chromatography-tandem mass spectrometry |
| spellingShingle | PAN Ying LV Shenliang XIA Sujie CHEN Yan XU Weidong Determination of 9 yellow colorants in fish tissues by ultra performance liquid chromatography-tandem mass spectrometry Zhongguo shipin weisheng zazhi fish tissues yellow colorants ultra high-performance liquid chromatography-tandem mass spectrometry |
| title | Determination of 9 yellow colorants in fish tissues by ultra performance liquid chromatography-tandem mass spectrometry |
| title_full | Determination of 9 yellow colorants in fish tissues by ultra performance liquid chromatography-tandem mass spectrometry |
| title_fullStr | Determination of 9 yellow colorants in fish tissues by ultra performance liquid chromatography-tandem mass spectrometry |
| title_full_unstemmed | Determination of 9 yellow colorants in fish tissues by ultra performance liquid chromatography-tandem mass spectrometry |
| title_short | Determination of 9 yellow colorants in fish tissues by ultra performance liquid chromatography-tandem mass spectrometry |
| title_sort | determination of 9 yellow colorants in fish tissues by ultra performance liquid chromatography tandem mass spectrometry |
| topic | fish tissues yellow colorants ultra high-performance liquid chromatography-tandem mass spectrometry |
| url | http://www.zgspws.com/zgspwszz/article/abstract/20220109?st=article_issue |
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