Sex- and age-specific reference intervals for diagnostic ratios reflecting relative activity of steroidogenic enzymes and pathways in adults.
<h4>Objective</h4>Diagnostic ratios calculated from urinary steroid hormone metabolites are used as a measure for the relative activity of steroidogenic enzymes or pathways in the clinical investigation of steroid metabolism disorders. However, population-based sex- and age-specific refe...
| Main Authors: | , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2021-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0253975&type=printable |
| _version_ | 1826554656055099392 |
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| author | Valentin Rousson Daniel Ackermann Belen Ponte Menno Pruijm Idris Guessous Claudia H d'Uscio Georg Ehret Geneviève Escher Antoinette Pechère-Bertschi Michael Groessl Pierre-Yves Martin Michel Burnier Bernhard Dick Murielle Bochud Bruno Vogt Nasser A Dhayat |
| author_facet | Valentin Rousson Daniel Ackermann Belen Ponte Menno Pruijm Idris Guessous Claudia H d'Uscio Georg Ehret Geneviève Escher Antoinette Pechère-Bertschi Michael Groessl Pierre-Yves Martin Michel Burnier Bernhard Dick Murielle Bochud Bruno Vogt Nasser A Dhayat |
| author_sort | Valentin Rousson |
| collection | DOAJ |
| description | <h4>Objective</h4>Diagnostic ratios calculated from urinary steroid hormone metabolites are used as a measure for the relative activity of steroidogenic enzymes or pathways in the clinical investigation of steroid metabolism disorders. However, population-based sex- and age-specific reference intervals and day-night differences in adults are lacking.<h4>Methods</h4>Sixty-five diagnostic ratios were calculated from steroid metabolites measured by GC-MS in day- and night-time and in 24-hour urine from 1128 adults recruited within the Swiss Kidney Project on Genes in Hypertension (SKIPOGH), a population-based, multicenter cohort study. Differences related to sex, age and day- and night-time were evaluated and reference curves in function of age and sex were modelled by multivariable linear mixed regression for diagnostic ratios and were compared to values from the literature.<h4>Results</h4>Most ratios had sex- and age-specific relationships. For each ratio, percentiles were plotted in function of age and sex in order to create reference curves and sex- and age-specific reference intervals derived from 2.5th and 97.5th percentiles were obtained. Most ratios reflected a higher enzyme activity during the day compared to the night.<h4>Conclusions</h4>Sex- and age-specific references for 24 hours, day and night urine steroid metabolite ratios may help distinguishing between health and disease when investigating human disorders affecting steroid synthesis and metabolism. The day-night differences observed for most of the diagnostic ratios suggest a circadian rhythm for enzymes involved in human steroid hormones metabolism. |
| first_indexed | 2024-12-19T04:33:05Z |
| format | Article |
| id | doaj.art-dcdebe85ad7d48c2b4293cbd39dce1a8 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2025-03-14T07:44:22Z |
| publishDate | 2021-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-dcdebe85ad7d48c2b4293cbd39dce1a82025-03-03T05:33:35ZengPublic Library of Science (PLoS)PLoS ONE1932-62032021-01-01167e025397510.1371/journal.pone.0253975Sex- and age-specific reference intervals for diagnostic ratios reflecting relative activity of steroidogenic enzymes and pathways in adults.Valentin RoussonDaniel AckermannBelen PonteMenno PruijmIdris GuessousClaudia H d'UscioGeorg EhretGeneviève EscherAntoinette Pechère-BertschiMichael GroesslPierre-Yves MartinMichel BurnierBernhard DickMurielle BochudBruno VogtNasser A Dhayat<h4>Objective</h4>Diagnostic ratios calculated from urinary steroid hormone metabolites are used as a measure for the relative activity of steroidogenic enzymes or pathways in the clinical investigation of steroid metabolism disorders. However, population-based sex- and age-specific reference intervals and day-night differences in adults are lacking.<h4>Methods</h4>Sixty-five diagnostic ratios were calculated from steroid metabolites measured by GC-MS in day- and night-time and in 24-hour urine from 1128 adults recruited within the Swiss Kidney Project on Genes in Hypertension (SKIPOGH), a population-based, multicenter cohort study. Differences related to sex, age and day- and night-time were evaluated and reference curves in function of age and sex were modelled by multivariable linear mixed regression for diagnostic ratios and were compared to values from the literature.<h4>Results</h4>Most ratios had sex- and age-specific relationships. For each ratio, percentiles were plotted in function of age and sex in order to create reference curves and sex- and age-specific reference intervals derived from 2.5th and 97.5th percentiles were obtained. Most ratios reflected a higher enzyme activity during the day compared to the night.<h4>Conclusions</h4>Sex- and age-specific references for 24 hours, day and night urine steroid metabolite ratios may help distinguishing between health and disease when investigating human disorders affecting steroid synthesis and metabolism. The day-night differences observed for most of the diagnostic ratios suggest a circadian rhythm for enzymes involved in human steroid hormones metabolism.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0253975&type=printable |
| spellingShingle | Valentin Rousson Daniel Ackermann Belen Ponte Menno Pruijm Idris Guessous Claudia H d'Uscio Georg Ehret Geneviève Escher Antoinette Pechère-Bertschi Michael Groessl Pierre-Yves Martin Michel Burnier Bernhard Dick Murielle Bochud Bruno Vogt Nasser A Dhayat Sex- and age-specific reference intervals for diagnostic ratios reflecting relative activity of steroidogenic enzymes and pathways in adults. PLoS ONE |
| title | Sex- and age-specific reference intervals for diagnostic ratios reflecting relative activity of steroidogenic enzymes and pathways in adults. |
| title_full | Sex- and age-specific reference intervals for diagnostic ratios reflecting relative activity of steroidogenic enzymes and pathways in adults. |
| title_fullStr | Sex- and age-specific reference intervals for diagnostic ratios reflecting relative activity of steroidogenic enzymes and pathways in adults. |
| title_full_unstemmed | Sex- and age-specific reference intervals for diagnostic ratios reflecting relative activity of steroidogenic enzymes and pathways in adults. |
| title_short | Sex- and age-specific reference intervals for diagnostic ratios reflecting relative activity of steroidogenic enzymes and pathways in adults. |
| title_sort | sex and age specific reference intervals for diagnostic ratios reflecting relative activity of steroidogenic enzymes and pathways in adults |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0253975&type=printable |
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