Pseudorabies virus-induced expression and antiviral activity of type I or type III interferon depend on the type of infected epithelial cell
Type I and III Interferons (IFNs) are the initial antiviral cytokines produced in response to virus infection. These IFNs in turn bind to their respective receptors, trigger JAK-STAT signaling and induce the expression of IFN-stimulated genes (ISGs) to engage antiviral functions. Unlike the receptor...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-11-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2022.1016982/full |
| _version_ | 1797991089684086784 |
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| author | Yue Yin Jinglin Ma Cliff Van Waesberghe Bert Devriendt Herman W. Favoreel |
| author_facet | Yue Yin Jinglin Ma Cliff Van Waesberghe Bert Devriendt Herman W. Favoreel |
| author_sort | Yue Yin |
| collection | DOAJ |
| description | Type I and III Interferons (IFNs) are the initial antiviral cytokines produced in response to virus infection. These IFNs in turn bind to their respective receptors, trigger JAK-STAT signaling and induce the expression of IFN-stimulated genes (ISGs) to engage antiviral functions. Unlike the receptor for type I IFNs, which is broadly expressed, the expression of the type III IFN receptor is mainly confined to epithelial cells that line mucosal surfaces. Accumulating evidence has shown that type III IFNs may play a unique role in protecting mucosal surfaces against viral challenges. The porcine alphaherpesvirus pseudorabies virus (PRV) causes huge economic losses to the pig industry worldwide. PRV first replicates in the respiratory tract, followed by spread via neurons and via lymph and blood vessels to the central nervous system and internal organs, e.g. the kidney, lungs and intestinal tract. In this study, we investigate whether PRV triggers the expression of type I and III IFNs and whether these IFNs exert antiviral activity against PRV in different porcine epithelial cells: porcine kidney epithelial cells (PK-15), primary respiratory epithelial cells (PoREC) and intestinal porcine epithelial cells (IPEC-J2). We show that PRV triggers a multiplicity of infection-dependent type I IFN response and a prominent III IFN response in PK-15 cells, a multiplicity of infection-dependent expression of both types of IFN in IPEC-J2 cells and virtually no expression of either IFN in PoREC. Pretreatment of the different cell types with equal amounts of porcine IFN-λ3 (type III IFN) or porcine IFN-α (type I IFN) showed that IFN-α, but not IFN-λ3, suppressed PRV replication and spread in PK-15 cells, whereas the opposite was observed in IPEC-J2 cells and both types of IFN showed anti-PRV activity in PoREC cells, although the antiviral activity of IFN-α was more potent than that of IFN-λ3 in the latter cell type. In conclusion, the current data show that PRV-induced type I and III IFN responses and their antiviral activity depend to a large extent on the epithelial cell type used, and for the first time show that type III IFN displays antiviral activity against PRV in epithelial cells from the respiratory and particularly the intestinal tract. |
| first_indexed | 2024-04-11T08:46:40Z |
| format | Article |
| id | doaj.art-dced218e8f7e4972ac5a0d5215969343 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-04-11T08:46:40Z |
| publishDate | 2022-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-dced218e8f7e4972ac5a0d52159693432022-12-22T04:33:56ZengFrontiers Media S.A.Frontiers in Immunology1664-32242022-11-011310.3389/fimmu.2022.10169821016982Pseudorabies virus-induced expression and antiviral activity of type I or type III interferon depend on the type of infected epithelial cellYue YinJinglin MaCliff Van WaesbergheBert DevriendtHerman W. FavoreelType I and III Interferons (IFNs) are the initial antiviral cytokines produced in response to virus infection. These IFNs in turn bind to their respective receptors, trigger JAK-STAT signaling and induce the expression of IFN-stimulated genes (ISGs) to engage antiviral functions. Unlike the receptor for type I IFNs, which is broadly expressed, the expression of the type III IFN receptor is mainly confined to epithelial cells that line mucosal surfaces. Accumulating evidence has shown that type III IFNs may play a unique role in protecting mucosal surfaces against viral challenges. The porcine alphaherpesvirus pseudorabies virus (PRV) causes huge economic losses to the pig industry worldwide. PRV first replicates in the respiratory tract, followed by spread via neurons and via lymph and blood vessels to the central nervous system and internal organs, e.g. the kidney, lungs and intestinal tract. In this study, we investigate whether PRV triggers the expression of type I and III IFNs and whether these IFNs exert antiviral activity against PRV in different porcine epithelial cells: porcine kidney epithelial cells (PK-15), primary respiratory epithelial cells (PoREC) and intestinal porcine epithelial cells (IPEC-J2). We show that PRV triggers a multiplicity of infection-dependent type I IFN response and a prominent III IFN response in PK-15 cells, a multiplicity of infection-dependent expression of both types of IFN in IPEC-J2 cells and virtually no expression of either IFN in PoREC. Pretreatment of the different cell types with equal amounts of porcine IFN-λ3 (type III IFN) or porcine IFN-α (type I IFN) showed that IFN-α, but not IFN-λ3, suppressed PRV replication and spread in PK-15 cells, whereas the opposite was observed in IPEC-J2 cells and both types of IFN showed anti-PRV activity in PoREC cells, although the antiviral activity of IFN-α was more potent than that of IFN-λ3 in the latter cell type. In conclusion, the current data show that PRV-induced type I and III IFN responses and their antiviral activity depend to a large extent on the epithelial cell type used, and for the first time show that type III IFN displays antiviral activity against PRV in epithelial cells from the respiratory and particularly the intestinal tract.https://www.frontiersin.org/articles/10.3389/fimmu.2022.1016982/fullepithelial cellpseudorabies virusalphaherpesvirustype I interferontype III interferonrespiratory |
| spellingShingle | Yue Yin Jinglin Ma Cliff Van Waesberghe Bert Devriendt Herman W. Favoreel Pseudorabies virus-induced expression and antiviral activity of type I or type III interferon depend on the type of infected epithelial cell Frontiers in Immunology epithelial cell pseudorabies virus alphaherpesvirus type I interferon type III interferon respiratory |
| title | Pseudorabies virus-induced expression and antiviral activity of type I or type III interferon depend on the type of infected epithelial cell |
| title_full | Pseudorabies virus-induced expression and antiviral activity of type I or type III interferon depend on the type of infected epithelial cell |
| title_fullStr | Pseudorabies virus-induced expression and antiviral activity of type I or type III interferon depend on the type of infected epithelial cell |
| title_full_unstemmed | Pseudorabies virus-induced expression and antiviral activity of type I or type III interferon depend on the type of infected epithelial cell |
| title_short | Pseudorabies virus-induced expression and antiviral activity of type I or type III interferon depend on the type of infected epithelial cell |
| title_sort | pseudorabies virus induced expression and antiviral activity of type i or type iii interferon depend on the type of infected epithelial cell |
| topic | epithelial cell pseudorabies virus alphaherpesvirus type I interferon type III interferon respiratory |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2022.1016982/full |
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